PMID: 8464723Mar 25, 1993Paper

Visualising the kinetics of dissociation of actinomycin from individual sites in mixed sequence DNA by DNase I footprinting

Nucleic Acids Research
M C Fletcher, K Fox

Abstract

We have investigated the kinetics of dissociation of actinomycin D from DNA by a variation of the footprinting technique. Complexes of actinomycin with a radiolabelled DNA fragment (tyrT) were dissociated by addition of a large excess of unlabelled calf thymus DNA and the mixture subjected to DNase I footprinting at subsequent intervals. The rates at which the footprints disappeared varied between the different binding sites. The dissociation was temperature dependent with average time constants of 30 s, 10 mins and 2 hours at temperatures of 37 degrees C, 20 degrees C and 4 degrees C respectively. The dissociation from a DNA fragment containing the synthetic insert T9GCA9 was significantly faster, with a half-life of about 1 min at 20 degrees C. In contrast, the dissociation of distamycin was too fast to measure (< 5 s) even at 4 degrees C.

References

May 20, 1975·Biochemistry·R Bittman, L Blau
May 20, 1992·Journal of Molecular Biology·S Kamitori, F Takusagawa
Jul 15, 1992·Biochimica Et Biophysica Acta·K Waterloh, K R Fox
Jul 28, 1968·Journal of Molecular Biology·W Müller, D M Crothers
Dec 17, 1984·European Journal of Biochemistry·K R Fox, M J Waring
Nov 28, 1983·FEBS Letters·A V Scamrov, R S Beabealashvilli
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M W Van DykeP B Dervan
Jul 27, 1981·Biochimica Et Biophysica Acta·K R Fox, M J Waring

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Citations

Apr 1, 1996·European Journal of Biochemistry·M C Fletcher, K R Fox
Nov 26, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Anupam DasAnjan Chakraborty
Jul 29, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Anupam DasAnjan Chakraborty
Mar 27, 2003·Journal of the American Chemical Society·Fredrik WesterlundPer Lincoln

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