Visualising the onset of viscous fingering in chromatography columns

Journal of Chromatography. a
Heather J CatchpooleG Guiochon

Abstract

Viscous fingering is an important fluid transport phenomenon that manifests itself when two fluids having different viscosities move in the same direction. Their interface is unstable and a complex fingering pattern may arise. This phenomenon is important in chromatography because it may lead to a decrease or even a failure in separations. The onset of viscous fingering was visually observed by packing a glass column with particles having the same refractive index as the mobile phase and injecting plugs of dye solutions having viscosities different from that of the mobile phase. Severe fingering effects are observed if the viscosity difference exceeds 0.17 cP. However, for smaller viscosity differences, band distortions are observed that may affect retention data, band efficiency, and band resolution. Careful attention should be paid to matching the mobile phase viscosity and that of the injection plug when accurate chromatographic information is required.

References

Aug 19, 2000·Journal of Chromatography. a·R A ShallikerG Guiochon

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Citations

Jun 7, 2012·Analytical and Bioanalytical Chemistry·David GodeDietrich A Volmer
Aug 21, 2013·Journal of Chromatography. a·Ross Andrew Shalliker, Harald Ritchie
Oct 19, 2011·Journal of Chromatography. a·G RousseauxA De Wit
Jan 8, 2010·Journal of Chromatography. a·Torgny Fornstedt
Mar 14, 2008·Journal of Chromatography. a·Georges GuiochonR Andrew Shalliker
Apr 11, 2007·Journal of Chromatography. a·G RousseauxM Martin
Feb 20, 2007·Journal of Separation Science·Eric Loeser, Patrick Drumm
Oct 13, 2006·Journal of Chromatography. a·R Andrew ShallikerGeorges Guiochon
Aug 16, 2006·Journal of Chromatography. a·Georges Guiochon
Apr 28, 2009·Analytica Chimica Acta·Isabelle FrançoisPat Sandra
Mar 5, 2015·Journal of Separation Science·Danielle N BassanesePaul G Stevenson
Dec 8, 2014·The Journal of Chemical Physics·Chinar Rana, Manoranjan Mishra
Aug 17, 2014·Journal of Chromatography. a·Jelle De VosSebastiaan Eeltink
Mar 27, 2012·Journal of Chromatography. a·Bradley J VanMiddlesworth, John G Dorsey
May 24, 2015·Journal of Chromatography. a·Martin EnmarkTorgny Fornstedt
Mar 24, 2015·Journal of Chromatography. a·Yun DaiArvind Rajendran
Feb 6, 2020·Journal of Separation Science·A Schultze-JenaA van der Padt
Apr 23, 2019·Chemical Engineering & Technology·Jari HeinonenTuomo Sainio
Apr 16, 2020·Physical Review. E·Chinar Rana, Manoranjan Mishra
Mar 5, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·M MishraA De Wit
May 22, 2013·Journal of Chromatography. a·Manoranjan MishraMichel Martin

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