Visualization and sensing of intermolecular interactions with two-color fluorescent probes

FEBS Letters
Alexander P Demchenko

Abstract

We developed a new generic fluorescence sensing technology based on the change of relative intensities between two well-separated emission bands of the novel functional 3-hydroxychromone (3HC) dyes. A greatly enhanced self-calibrating wavelength-ratiometric response is obtained to all major types of non-covalent interactions that can be used in sensing--to polarity, hydrogen bonding ability and to local electrostatic fields. This technology may find a broad range of applications--from homogeneous assays in solutions to microarrays, microfluidic devices, nanosensors and whole cell imaging systems. It allows transforming micelles or phospholipid vesicles into nanosensor devices. In cellular research a high sensitivity to membrane potentials can be obtained and the membrane changes during apoptosis detected.

References

Apr 7, 1998·Biochimica Et Biophysica Acta·O P BondarE S Rowe
Jan 7, 2000·Annals of Biomedical Engineering·J J PancrazioD A Stenger
Aug 5, 1997·Chemical Reviews·A. Prasanna de SilvaTerence E. Rice
Jun 8, 2002·Journal of Molecular Biology·Martial RenardHugues Bedouelle
Oct 10, 2002·Journal of the American Chemical Society·Andrey S Klymchenko, Alexander P Demchenko
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Robert M de LorimierHomme W Hellinga
Nov 26, 2002·Chemistry & Biology·Andrey S KlymchenkoAlexander P Demchenko
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Andrey S KlymchenkoAlexander P Demchenko
Apr 28, 2004·Biophysical Journal·Andrey S KlymchenkoYves Mély
May 12, 2004·Analytical Biochemistry·Andrey S KlymchenkoAlexander P Demchenko
May 3, 2005·International Journal of Biological Macromolecules·Sebnem ErcelenAlexander P Demchenko
Jun 1, 2005·Biochimica Et Biophysica Acta·Vasyl V ShynkarYves Mély
Jun 22, 2005·Trends in Biotechnology·Alexander P Demchenko
Jul 16, 2005·Analytical Biochemistry·Alexander P Demchenko
Jul 16, 2005·Journal of Biochemical and Biophysical Methods·Zeynep TurkmenAlexander P Demchenko

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Citations

Jul 17, 2012·Cytotechnology·Alexander P Demchenko
Apr 2, 2010·Journal of Fluorescence·Alexander P Demchenko
Jul 14, 2011·Journal of the American Chemical Society·Youngmin YouStephen J Lippard
Jun 2, 2009·Chemistry and Physics of Lipids·Alexander P Demchenko, Semen O Yesylevskyy
Nov 12, 2015·Journal of Photochemistry and Photobiology. B, Biology·Asli CapanTuran Ozturk
Sep 8, 2015·Journal of Fluorescence·Venelin EnchevNadezhda Markova
Apr 30, 2016·Journal of Materials Chemistry. C, Materials for Optical and Electronic Devices·Mark A Haidekker, Emmanuel A Theodorakis
May 1, 2013·Methods and Applications in Fluorescence·Alexander P Demchenko
May 3, 2018·Beilstein Journal of Organic Chemistry·Márton BojtárIstván Bitter
Apr 15, 2015·The Journal of Biological Chemistry·Nikita A KuznetsovOlga S Fedorova
Nov 2, 2014·Angewandte Chemie·Alexander P DemchenkoJuan Carlos Del Valle
Nov 22, 2012·Chemical Society Reviews·Alexander P DemchenkoPi-Tai Chou
May 24, 2007·Journal of the American Chemical Society·Matthew S TremblayDalibor Sames
Dec 14, 2016·Chemical Reviews·Tatu KumpulainenEric Vauthey
Dec 18, 2019·Journal of the American Chemical Society·Soumit ChatterjeePeter Karuso

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis