Visualization by high resolution immunoelectron microscopy of the transient receptor potential vanilloid-1 at inhibitory synapses of the mouse dentate gyrus

PloS One
Miren-Josune CanduelaP Grandes

Abstract

We have recently shown that the transient receptor potential vanilloid type 1 (TRPV1), a non-selective cation channel in the peripheral and central nervous system, is localized at postsynaptic sites of the excitatory perforant path synapses in the hippocampal dentate molecular layer (ML). In the present work, we have studied the distribution of TRPV1 at inhibitory synapses in the ML. With this aim, a preembedding immunogold method for high resolution electron microscopy was applied to mouse hippocampus. About 30% of the inhibitory synapses in the ML are TRPV1 immunopositive, which is mostly localized perisynaptically (∼60% of total immunoparticles) at postsynaptic dendritic membranes receiving symmetric synapses in the inner 1/3 of the layer. This TRPV1 pattern distribution is not observed in the ML of TRPV1 knock-out mice. These findings extend the knowledge of the subcellular localization of TRPV1 to inhibitory synapses of the dentate molecular layer where the channel, in addition to excitatory synapses, is present.

References

Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Susan M HuangVincenzo Di Marzo
Apr 29, 2005·Brain Research. Molecular Brain Research·Attila TóthPeter M Blumberg
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yoshinobu KawamuraMasanobu Kano
Jan 26, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rudolph MarschCarsten T Wotjak
Mar 14, 2007·Pharmacology & Therapeutics·Katarzyna StarowiczVincenzo Di Marzo
Feb 1, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Rita NyilasIstván Katona
Apr 15, 2008·Biological Psychiatry·Hong-Bin LiLin Xu
Jun 27, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Vincenzo MicaleVincenzo Di Marzo
Sep 9, 2008·Nature Medicine·István Katona, Tamás F Freund
Mar 17, 2009·Trends in Neurosciences·Julie A Kauer, Helen E Gibson
May 2, 2009·The Journal of Physiology·Sabatino MaioneVincenzo Di Marzo
Nov 16, 2010·Nature Neuroscience·Andrés E ChávezPablo E Castillo
Nov 16, 2010·Nature Neuroscience·Brad A GrueterRobert C Malenka
Apr 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel J CavanaughAllan I Basbaum
Nov 8, 2011·Nature Neuroscience·Nagore PuenteOlivier J Manzoni
Jan 21, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Francesco MoriDiego Centonze
Apr 25, 2012·Annual Review of Neuroscience·István Katona, Tamás F Freund
Dec 17, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrés E ChávezPablo E Castillo

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Citations

Sep 29, 2017·Physiological Reviews·Kenneth A PelkeyChris J McBain
Nov 19, 2020·International Journal of Molecular Sciences·Cecilia Zavala-TecuapetlaHiram Luna-Munguia

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