Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging

Scientific Reports
Yoonhee KimDo Young Noh

Abstract

We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm3. Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.

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Citations

Jun 15, 2019·Nature Communications·Gisela BrändénRichard Neutze
May 10, 2020·Journal of Synchrotron Radiation·Yoonhee KimDo Young Noh
Mar 3, 2021·Journal of Synchrotron Radiation·Zhongzhu ZhuLiang Zhou
Dec 24, 2021·Molecular Metabolism·Valentina Loconte, Kate L White

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Methods Mentioned

BETA
electron microscopies
x-ray diffraction
scanning electron microscopy
chip
AFM
atomic force microscopy
fluorescence microscopy
electron

Software Mentioned

CXDI

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