Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts

Science
Petr KalabRebecca Heald

Abstract

The small guanosine triphosphatase Ran is loaded with guanosine triphosphate (GTP) by the chromatin-bound guanine nucleotide exchange factor RCC1 and releases import cargoes in the nucleus during interphase. In mitosis, Ran-GTP promotes spindle assembly around chromosomes by locally discharging cargoes that regulate microtubule dynamics and organization. We used fluorescence resonance energy transfer-based biosensors to visualize gradients of Ran-GTP and liberated cargoes around chromosomes in mitotic Xenopus egg extracts. Both gradients were required to assemble and maintain spindle structure. During interphase, Ran-GTP was highly enriched in the nucleoplasm, and a steep concentration difference between nuclear and cytoplasmic Ran-GTP was established, providing evidence for a Ran-GTP gradient surrounding chromosomes throughout the cell cycle.

References

Jul 21, 1998·Science·R Y Tsien, A Miyawaki
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
May 14, 1999·Current Biology : CB·P KalabM Dasso
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D Görlich, U Kutay
Jul 27, 2000·Molecular Cell·M HetzerI W Mattaj
Jul 13, 2001·The Journal of Biological Chemistry·B CatimelB Kobe

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Citations

Jan 8, 2005·Chembiochem : a European Journal of Chemical Biology·Anna PeykerPhilippe I H Bastiaens
Feb 20, 2008·Journal of Mathematical Biology·Jörg Stelling, Boris N Kholodenko
Oct 21, 2010·Plant Cell Reports·Thomas Merkle
Sep 1, 2005·Chromosoma·Jennifer R Ball, Katharine S Ullman
Sep 11, 2010·Protoplasma·Patricia WadsworthTobias I Baskin
Sep 14, 2006·Pharmaceutical Research·James R DavisCarol S Lim
Oct 21, 2010·Virologica Sinica·Qiong DingAlan C Zheng
Oct 24, 2003·Trends in Cell Biology·Boris M SlepchenkoLeslie M Loew
Oct 24, 2003·Trends in Cell Biology·Hoi-Yeung LiYixian Zheng
May 26, 2004·Methods : a Companion to Methods in Enzymology·Minoru Yoshida, Shelley Sazer
Jan 9, 2003·Current Opinion in Cell Biology·François NédélecEric Karsenti
Jun 6, 2003·Current Opinion in Cell Biology·B Booth Quimby, Mary Dasso
Jul 11, 2003·Developmental Cell·Alexei Arnaoutov, Mary Dasso
Apr 8, 2011·ACS Chemical Biology·Jonathan F SoderholmRebecca Heald
Mar 2, 2005·Nature Cell Biology·Jody Rosenblatt
Jun 2, 2005·Nature Cell Biology·Paul R Clarke
Sep 2, 2006·Nature Cell Biology·Isabelle Vernos
Feb 17, 2006·Nature Reviews. Molecular Cell Biology·Boris N Kholodenko
May 15, 2008·Nature Reviews. Molecular Cell Biology·Paul R Clarke, Chuanmao Zhang
May 25, 2010·Nature Reviews. Molecular Cell Biology·Boris N KholodenkoWalter Kolch
May 21, 2010·Nature Reviews. Molecular Cell Biology·Leif Dehmelt, Philippe I H Bastiaens
May 24, 2011·Nature Reviews. Molecular Cell Biology·Jonathon HowardJustin S Bois
Sep 24, 2011·Nature Reviews. Molecular Cell Biology·Asifa AkhtarMarino Zerial
Jan 6, 2012·Molecular BioSystems·Mikhail A TsyganovBoris N Kholodenko
Oct 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shotaro OtsukaShige H Yoshimura
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jiong Ma, Weidong Yang
Jun 26, 2009·The Journal of Biological Chemistry·Kai YuanXuebiao Yao
Dec 14, 2011·The Journal of Biological Chemistry·Rafael PeláezFernando Moreno
Mar 19, 2008·The Journal of Biological Chemistry·Harald SchulzeRamesh A Shivdasani
Jun 13, 2003·Molecular Biology of the Cell·James A Galbraith, Mark Terasaki
Oct 1, 2003·Molecular Biology of the Cell·Guy KeryerAnne-Marie Tassin
Oct 1, 2003·Molecular Biology of the Cell·Chad G PearsonKerry Bloom
Oct 11, 2003·Molecular Biology of the Cell·Amnon HarelDouglass J Forbes
Sep 19, 2003·Molecular Biology of the Cell·Stephanie C Ems-McClungClaire E Walczak
Feb 10, 2004·Molecular Biology of the Cell·Jennifer S TirnauerTimothy J Mitchison
Apr 9, 2005·Molecular Biology of the Cell·Sten StrunzeUrs F Greber
Jun 30, 2006·Molecular Biology of the Cell·Arturo V OrjaloDouglass J Forbes
Dec 22, 2006·Molecular Biology of the Cell·Kathryn J RyanSusan R Wente

❮ Previous
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