Oct 27, 2018

Visualization of axonal protein allocation in Drosophila with whole brain localization microscopy

BioRxiv : the Preprint Server for Biology
Li-An ChuAnn-Shyn Chiang

Abstract

Long-term memory (LTM) formation requires learning-induced protein synthesis in specific neurons and synapses within a neural circuit. Precisely how neuronal activity allocates new proteins to specific synaptic ensembles, however, remains unknown. We developed a deep-tissue super-resolution imaging tool suitable for single-molecule localization in intact adult Drosophila brain, and focused on the axonal protein allocation in mushroom body (MB), a central neuronal structure involved in olfactory memory formation. We found that insufficient training suppresses LTM formation by inducing the synthesis of vesicular monoamine transporter (VMAT) proteins within a dorsal paired medial (DPM) neuron, which innervates all axonal lobes of the MB. Surprisingly, using our localization microscopy, we found that these learning-induced proteins are distributed only in a subset of DPM axons in specific sectors along the MB lobes. This neural architecture suggest that sector-specific modulation of neuronal activity from MB neurons gates consolidation of early transient memory into LTM.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Translational Activation
Neuron Structure
SLC18A1
Neurons
Brain
Memory, Long-Term
Drosophila
Smell Perception
Procedure on Brain

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