Visualization of Calcium Dynamics in Kidney Proximal Tubules

Journal of the American Society of Nephrology : JASN
Kornélia SzebényiBalázs Sarkadi

Abstract

Intrarenal changes in cytoplasmic calcium levels have a key role in determining pathologic and pharmacologic responses in major kidney diseases. However, cell-specific delivery of calcium-sensitive probes in vivo remains problematic. We generated a transgenic rat stably expressing the green fluorescent protein-calmodulin-based genetically encoded calcium indicator (GCaMP2) predominantly in the kidney proximal tubules. The transposon-based method used allowed the generation of homozygous transgenic rats containing one copy of the transgene per allele with a defined insertion pattern, without genetic or phenotypic alterations. We applied in vitro confocal and in vivo two-photon microscopy to examine basal calcium levels and ligand- and drug-induced alterations in these levels in proximal tubular epithelial cells. Notably, renal ischemia induced a transient increase in cellular calcium, and reperfusion resulted in a secondary calcium load, which was significantly decreased by systemic administration of specific blockers of the angiotensin receptor and the Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacolog...Continue Reading

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Citations

Feb 29, 2016·Cell Calcium·Ágota ApátiBalázs Sarkadi
Oct 9, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Katalin JemnitzZsuzsa Veres
Oct 12, 2016·Scientific Reports·Jung Eun ParkAfonso C Silva
Feb 16, 2017·Current Opinion in Nephrology and Hypertension·Andrew M HallDominik Haenni
Oct 19, 2017·Physiological Reports·Sohinee BhattacharyyaOra A Weisz
Dec 10, 2016·Disease Models & Mechanisms·Linda J MullinsJohn J Mullins
Mar 24, 2016·Current Opinion in Nephrology and Hypertension·János Peti-PeterdiAnne Riquier-Brison
Jun 23, 2020·American Journal of Physiology. Renal Physiology·Joana MartinsAndrew M Hall
Sep 22, 2015·Scientific Reports·Ming ZhaoLeilei Peng
Nov 28, 2020·The American Journal of Pathology·Sun-Ji ParkYing Maggie Chen
Jan 3, 2018·Seminars in Nephrology·Georgina GyarmatiJános Peti-Peterdi
May 21, 2021·Kidney International·Joana R MartinsAndrew M Hall

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