Visualization of calcium influx through channels that shape the burst and tonic firing modes of thalamic relay cells

Journal of Neurophysiology
Q ZhouP R Adams

Abstract

Thalamic neurons have two firing modes: "tonic" and "burst." During burst mode, both low-threshold (LT) and high-threshold (HT) calcium channels are activated, while in tonic mode, only the HT-type of calcium channel is activated. The calcium signals associated with each firing mode were investigated in rat thalamic slices using whole cell patch clamping and confocal calcium imaging. Action potentials were induced by direct current injection into thalamic relay cells loaded with a fluorescent calcium indicator. In both tonic and burst firing modes, large calcium signals were recorded throughout the soma and proximal dendrites. To map the distribution of the channels mediating these calcium fluxes, LT and HT currents were independently activated using specific voltage-clamp protocols. We focused on the proximal region of the cell (up to 50 microm from the soma) because it appeared to be well clamped. For a voltage pulse of a given size, the largest calcium signals were observed in the proximal dendrites with smaller signals occurring in the soma and nucleus. This was true for both LT and HT signals. Rapid imaging, using one-dimensional linescans, was used to more precisely localize the calcium influx. For both LT and HT channels...Continue Reading

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