PMID: 7537077Dec 1, 1994Paper

Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis

International Journal of Biological Macromolecules
P BellemannK Geider

Abstract

Exopolysaccharide (EPS) synthesis by Erwinia amylovora depends on environmental and genetic predispositions. To measure the amount of the acidic EPS amylovoran synthesized by E. amylovora cell cultures, a turbidity assay using cetylpyridinium salt was developed. The EPS produced by bacteria grown on solid media was additionally characterized by its water content. The amylovoran capsules were visualized in situ by staining with fluorescein isothiocyanate (FITC)-labelled lectin from Abrus precatorius, which reacts with the galactose residue of the EPS side chain. The staining and the turbidity assays were applied to suspension cell cultures or to cells from colonies and did not require any purification steps. Lectin staining was superior to electron microscopic (EM) techniques for visualization of capsules. For EM, the capsule was stabilized with polycationic ferritin. In contrast to lectin staining, only a small fraction of the cells was found to be EPS-coated in the EM assay. An increase in capsulation and in amylovoran production was found in conjunction with mutations in a ribosomal protein conferring resistance to streptomycin. Furthermore, the presence of sorbitol in the growth environment resulted in high synthesis of amyl...Continue Reading

Citations

Sep 18, 2004·Plant Cell Reports·Mickaël MalnoyElisabeth Chevreau
Jan 13, 2006·Molecular Genetics and Genomics : MGG·M HildebrandK Geider
Mar 19, 2013·Applied and Environmental Microbiology·Dwayne R RoachAntonet M Svircev
Aug 9, 2011·Applied and Environmental Microbiology·Jessica M KoczanGeorge W Sundin
Mar 12, 2013·Journal of Bacteriology·Adam C EdmundsChristopher M Waters
Sep 3, 2014·Archives of Microbiology·Emadeldeen IsmailJoanna Puławska
Jun 10, 2015·International Journal of Molecular Sciences·Núria PiquéJuan M Tomás
Feb 25, 2009·Molecular Plant Pathology·Dongping WangYoufu Zhao
Sep 13, 2003·Letters in Applied Microbiology·R K Taylor, C N Hale
Feb 11, 2015·Journal of Bacteriology·Veronica AnconaYoufu Zhao
Oct 28, 2018·Applied and Environmental Microbiology·Roshni R KharadiGeorge W Sundin
Mar 20, 2019·Journal of Bacteriology·Sara M KleeTimothy W McNellis

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