Visualization of cellulose synthase demonstrates functional association with microtubules

Science
Alexander R ParedezDavid W Ehrhardt

Abstract

Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic Arabidopsis plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton.

References

Jan 19, 2000·Trends in Plant Science·A M Emons, B M Mulder
Jun 2, 2001·The Journal of Biological Chemistry·O GriesbeckR Y Tsien
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·W R ScheibleC Somerville
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·M YuanC W Lloyd
Jul 30, 2002·The Journal of Biological Chemistry·Joséphine Lai-Kee-HimVincent Bulone
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Neil G TaylorSimon R Turner
Apr 26, 2003·Science·Sidney L ShawDavid W Ehrhardt
Aug 5, 2003·The Plant Cell·John C GardinerSimon R Turner
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Regina HimmelspachGeoffrey O Wasteneys
Oct 20, 2004·Current Opinion in Plant Biology·Geoffrey O Wasteneys
Dec 25, 2004·Science·Chris SomervilleHeather Youngs
Dec 28, 1962·Science·P B Green
Oct 1, 1963·The Journal of Cell Biology·M C Ledbetter, K R Porter

❮ Previous
Next ❯

Citations

Jan 21, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Doug J HinchliffeBarbara A Triplett
Jun 18, 2009·Protoplasma·Michael VolggerIrene Lichtscheidl
Oct 19, 2011·Protoplasma·Peter Nick
Jan 26, 2007·Journal of Plant Research·Yoshinobu Mineyuki
Dec 31, 2009·Bulletin of Mathematical Biology·F DiotalleviJ Grasman
May 15, 2013·Trends in Plant Science·Roman PleskotChristopher J Staiger
May 25, 2007·Nature Reviews. Molecular Cell Biology·G Venugopala ReddyElliot M Meyerowitz
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ying GuChris Somerville
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ying GuChris R Somerville
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shaolin ChenChris R Somerville
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Siyuan WangJoshua W Shaevitz
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shundai LiYing Gu
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Darby M HarrisSeth Debolt
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sidney L Shaw
Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Okako OmadjelaJochen Zimmer
May 23, 2008·Journal of Experimental Botany·Jian WangRichard E Williamson
Oct 4, 2008·Journal of Experimental Botany·Jana HofmannováZdenek Opatrny
Oct 24, 2008·Journal of Experimental Botany·Grantley Lycett
Aug 19, 2009·Journal of Experimental Botany·Purbasha SarkarManfred Auer
Feb 18, 2010·Journal of Experimental Botany·Christopher J StaigerLaurent Blanchoin
Aug 25, 2011·Journal of Experimental Botany·Rosemary G White, Deborah A Barton
Mar 1, 2012·Journal of Experimental Botany·Dirk De VosGerrit T S Beemster
Feb 3, 2009·Plant & Cell Physiology·Dyani LewisEdward J Newbigin
Feb 4, 2009·Plant & Cell Physiology·Raymond WightmanSimon R Turner
Jan 9, 2010·Plant & Cell Physiology·Satoshi FujiiKoichi Mizuno
Sep 7, 2011·Plant & Cell Physiology·Miriam AkkermanTijs Ketelaar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.