PMID: 8939774Dec 1, 1996Paper

Visualization of crystal-matrix structure. In situ demineralization of mineralized turkey leg tendon and bone

Calcified Tissue International
K S Prostak, S Lees

Abstract

A technique to correlate the ultrastructural distribution of mineral with its organic material in identical sections of mineralized turkey leg tendon (MTLT) and human bone was developed. Osmium or ethanol fixed tissues were processed for transmission electron microscopy (TEM). The mineralized tissues were photographed at high, intermediate, and low magnifications, making note of section features such as fibril geometry, colloidal gold distribution, or section artifacts for subsequent specimen realignment after demineralization. The specimen holder was removed from the microscope, the tissue section demineralized in situ with a drop of 1 N HCl, then stained with 2% aqueous vanadyl sulfate. The specimen holder was reinserted into the microscope, realigned with the aid of the section features previously noted, and rephotographed at identical magnification used for the mineralized sections. A one to one correspondence was apparent between the mineral and its demineralized crystal "ghost" in both MTLT and bone. The fine structural periodic banding seen in unmineralized collagen was not observed in areas that were fully mineralized before demineralization, indicating that the axial arrangement of the collagen molecules is altered sig...Continue Reading

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Citations

Feb 14, 2007·Biomechanics and Modeling in Mechanobiology·Young June Yoon, Stephen C Cowin
Feb 6, 2010·Biomechanics and Modeling in Mechanobiology·Andreas G ReisingerPhilippe K Zysset
Jul 24, 1998·Journal of Biomechanical Engineering·S WeissA Meunier
Jan 1, 2000·The Journal of the Acoustical Society of America·T D MastR C Waag
Dec 17, 2015·International Journal for Numerical Methods in Biomedical Engineering·Romane BlanchardChristian Hellmich
Mar 24, 2017·Journal of Biomechanical Engineering·Diab W AbueiddaIwona M Jasiuk
Sep 10, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elia Beniash
May 9, 2012·Computer Methods in Biomechanics and Biomedical Engineering·Lukas EberhardsteinerStefan Scheiner
Feb 9, 2016·Interface Focus·Lester J SmithStavros Thomopoulos
Apr 4, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lisa M MillerStefan Judex
Dec 28, 2019·Materials·Amadeus C S de AlcântaraPaulo Sollero
Dec 29, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Jitin SamuelXiaodu Wang
Jun 2, 2021·Scientific Reports·Fabiano BiniFranco Marinozzi

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