PMID: 8945928Nov 1, 1996Paper

Visualization of doxorubicin-induced oxidative stress in isolated cardiac myocytes

The American Journal of Physiology
N Sarvazyan

Abstract

The paper presents high-resolution fluorescence images obtained using laser-scanning confocal microscopy. Isolated cells from adult rat hearts were preloaded with 2',7'-dichlorofluorescin (an oxidant-sensitive fluorescent probe) and exposed to doxo- rubicin, an important anticancer drug with prominent cardioxicity. Fluorescence images were collected from live cells simultaneously on two channels: 1) 515-530 nm emission range was used to monitor an increase in dichlorofluorescein, the oxidized product of dichlorofluorescein, and 2) emission > 610 nm was used to visualize the intracellular distribution of doxorubicin. The images reveal intracellular oxidation close to the mitochondria after only 20 min of exposure of isolated cardiomyocytes to 40-160 microM doxorubicin. The data confirm an oxidative mechanism of doxorubicin cardiotoxicity and demonstrate the capability of a new technique to monitor intracellular oxidation in living cardiomyocytes.

Citations

Mar 28, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Thomas L'EcuyerRichard Vander Heide
Jan 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Thomas L'EcuyerRichard Vander Heide
May 11, 2010·Amino Acids·Aaron K HolleyDaret K St Clair
Jul 24, 2010·Annals of the New York Academy of Sciences·Aaron K HolleyDaret K St Clair
May 11, 2018·Oxidative Medicine and Cellular Longevity·Stefania GoriniElisabetta Ferraro
Apr 1, 2020·Frontiers in Cardiovascular Medicine·Alessandra MurabitoAlessandra Ghigo
Mar 10, 2000·American Journal of Physiology. Heart and Circulatory Physiology·L M Swift, N Sarvazyan
Nov 11, 2011·International Journal of Molecular Sciences·Aaron K HolleyDaret K St Clair
Jun 27, 2019·International Journal of Molecular Sciences·Giulia QuerioMaria Pia Gallo
Nov 5, 2020·Oxidative Medicine and Cellular Longevity·James H Doroshow
May 15, 2002·Journal of Photochemistry and Photobiology. B, Biology·Ludovic BourréThierry Patrice

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