Visualization of dynamic change in contraction-induced lipid composition in mouse skeletal muscle by matrix-assisted laser desorption/ionization imaging mass spectrometry

Analytical and Bioanalytical Chemistry
Naoko Goto-InoueNobuharu L Fujii

Abstract

Lipids in skeletal muscle play a fundamental role both in normal muscle metabolism and in disease states. Skeletal muscle lipid accumulation is associated with several chronic metabolic disorders, including obesity, insulin resistance, and type 2 diabetes. However, it is poorly understood whether the lipid composition of skeletal muscle changes by contraction, due to the complexity of lipid molecular species. In this study, we used matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) to investigate changes in skeletal muscle lipid composition induced by contraction. We successfully observed the reduction of diacylglycerol and triacylglycerol, which are generally associated with muscle contraction. Interestingly, we found the accumulation of some saturated and mono-unsaturated fatty acids and poly-unsaturated fatty acids containing phosphatidylcholine in contracted muscles. Moreover, the distributions of several types of lipid were changed by contraction. Our results show that changes in the lipid amount, lipid composition, and energy metabolic activity can be evaluated in each local spot of cells and tissues at the same time using MALDI-IMS. In conclusion, MALDI-IMS is a powerful tool for studying l...Continue Reading

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Citations

Jan 15, 2013·The Analyst·David Gode, Dietrich A Volmer
Sep 1, 2014·The Journal of Surgical Research·Yasushi ShibasakiHiroyuki Konno
Dec 10, 2014·Analytical Chemistry·Bernhard Spengler
Jan 20, 2015·Molecular BioSystems·Chunyan WangXianlin Han
Oct 28, 2018·Rapid Communications in Mass Spectrometry : RCM·Naoko Goto-InoueTsukasa Mori
Oct 4, 2013·American Journal of Physiology. Cell Physiology·Licht MiyamotoTatsuya Hayashi
Jun 6, 2020·Mass Spectrometry Reviews·Enrico DavoliLavinia Morosi
Oct 16, 2015·Molecular and Cellular Biochemistry·Naoko Goto-InoueNobuharu L Fujii
Jan 6, 2021·Food & Function·Sakuka Tsukamoto-SenNobuharu L Fujii
Jun 27, 2019·Journal of Agricultural and Food Chemistry·Naoko Goto-InoueTsukasa Mori

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