Visualization of gene therapy with a liver cancer-targeted adeno-associated virus 3 vector

Journal of Cancer
Xusheng LiuZhijun Pei

Abstract

Background: To evaluate the feasibility of a self-complementing recombinant adeno-associated virus 3 (scrAAV3) vector targeting liver cancer and non-invasively monitor gene therapy of liver cancer. Materials and methods: An scrAAV3-HSV1-TK-kallistatin (ATK) gene drug was constructed, which contained the herpes virus thymidine kinase (HSV1-TK) reporter gene and human endogenous angiogenesis inhibitor (kallistatin) gene for non-invasive imaging of gene expression. Subcutaneous xenografted tumors of hepatoma in nude mice were generated for positron emission tomography/computed tomography (PET/CT) imaging. The ATK group was injected with the ATK gene through the tail vein, and an imaging agent was injected 2 weeks later. PET/CT imaging was performed at 1 hour after injection of the imaging agent. The control group was injected with phosphate-buffered saline at the same volume as the ATK gene drug. HE staining is used for pathological observation of tumor sections. HSV1-TK and kallistatin expression was identified by immunofluorescence, real-time quantitative PCR, and western blotting. Results: Radioactivity on PET/CT images was significantly higher in the ATK group compared with the control group. 18F-FHBG uptake values of left for...Continue Reading

Citations

Jan 22, 2021·Life Sciences·Esam Bashir Yahya, Amaal Mohammed Alqadhi
May 14, 2021·Molecular Therapy Oncolytics·Susanna C ConcilioKah-Whye Peng
Oct 29, 2021·Frontiers in Cell and Developmental Biology·Xu-Sheng LiuZhi-Jun Pei

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Methods Mentioned

BETA
xenograft
PCR
xenografts

Software Mentioned

SPSS

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