PMID: 2110588Jun 1, 1990Paper

Visualization of glycosaminoglycans in rat incisor predentin and dentin with cetylpyridinium chloride-glutaraldehyde as fixative

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
H ChardinM Goldberg

Abstract

Using cetylpyridinium chloride (CPC) in glutaraldehyde as fixative, we observed sinuous fiber-like structures 300-500 nm long and 7-14 nm thick in the spaces between the collagen fibers of rat incisor predentin. Small granules and fibrils were also detected. Electron-dense vesicles were seen inside the odontoblast processes. The plasma membrane was irregularly stained with material that adhered to its surface. In demineralized dentin, needle-like structures were seen at the periphery of globular structures which were not stained. Staining the sections with Alcian blue did not greatly improve the visualization of CPC-precipitated glycosaminoglycans. The specificity of staining was assessed on serial sections by selective dissociation of glycosaminoglycan aggregates with 2 M calcium chloride and their digestion by bovine testicular hyaluronidase. The glycosaminoglycans were probably combined with lipids, because treatment of the sections with a chloroform/methanol mixture removed the CPC-induced precipitates from both predentin and dentin.

Citations

Jun 1, 1994·Microscopy Research and Technique·T H van Kuppevelt, J H Veerkamp
Nov 1, 1993·The Histochemical Journal·M Goldberg, M Takagi
Mar 1, 1992·Calcified Tissue International·J R NefussiM Goldberg
Jul 1, 1993·Cell and Tissue Research·A GritliM Goldberg
Apr 20, 2005·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·A K S ArambawattaM Wakita
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·G EmberyM Goldberg
Apr 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J R NefussiN Forest
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A L Boskey

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