Visualization of interphase chromosomes in postmitotic cells of the human brain by multicolour banding (MCB).

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
Ivan Y IourovYuri B Yurov

Abstract

Molecular cytogenetics offers the unique possibility of investigating numerical and structural chromosomal aberrations in interphase nuclei of somatic cells. Previous fluorescence in-situ hybridization (FISH) investigations gave hints of numerical chromosomal imbalances in the human brain, present as low-level mosaicism. However, as precise identification of aneuploidy rates in somatic tissues faces major difficulties due to the limitations of FISH using whole chromosome painting or centromeric probes, in this study low-level mosaicism in the human brain was addressed for the first time using microdissection-based multicolour banding (MCB) probe sets. We demonstrated that MCB is suitable for this application and leads to more reliable results than the use of centromeric probes in parallel on the same samples. Autosomes and the active X chromosome appear as discrete metaphase chromosome-like structures, while the inactive X chromosome is condensed in more than 95% of interphase nuclei. The frequency of stochastic aneuploidy was found to be 0.2-0.5% (mean 0.35%) per autosome pair, 2% for the X chromosome in the female brain, and 0.4% in the male brain, giving a cumulative frequency of aneuploidy of approximately 10% in the adult ...Continue Reading

References

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Citations

May 8, 2007·Journal of Medical Genetics·Y B YurovN L Gorbachevskaya
May 31, 2011·Journal of Biomedical Science·Jasmine P DevadhasanJeongho An
Nov 27, 2008·Molecular Cytogenetics·Ivan Y IourovYuri B Yurov
Feb 26, 2010·Molecular Cytogenetics·Svetlana G VorsanovaIvan Y Iourov
Jul 31, 2010·Genome Medicine·Caroline RobberechtJoris Robert Vermeesch
Jun 28, 2007·PloS One·Yuri B YurovIlia V Soloviev
May 27, 2009·International Journal of Molecular Sciences·Thomas ArendtMarkus Morawski
Mar 8, 2014·Molecular Cytogenetics·Yuri B YurovIvan Y Iourov
Nov 19, 2008·Molecular Cytogenetics·Marina ManvelyanThomas Liehr
Mar 8, 2013·Seminars in Cell & Developmental Biology·Diane M Bushman, Jerold Chun
Nov 29, 2011·Clinics in Laboratory Medicine·Colleen Jackson-Cook
Apr 12, 2017·Journal of Neurochemistry·Thomas ArendtUwe Ueberham
Jul 22, 2018·Developmental Neurobiology·Suzanne RohrbackJerold Chun
Nov 20, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jyoti P ChaudhuriJoachim U Walther
Mar 26, 2019·FEBS Letters·Andrea Chicano, Joan-Ramon Daban
May 22, 2019·Genes·Ivan Y IourovSergei I Kutsev
Jun 15, 2021·Physical Biology·Joan-Ramon Daban

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