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Visualization of kinase activity with FRET-based activity biosensors

Current Protocols in Molecular Biology

Jun 29, 2010

Charlene Depry, Jin Zhang

Abstract

Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells...read more

Mentioned in this Paper

Biosensors
KARS gene
Immunofluorescence Microscopy
Biosensing Techniques
Phosphotransferases
Fluorescence Resonance Energy Transfer
Metazoa
Kinase Activity
Microscopy, Fluorescence
Paper Details
References
  • References9
  • Citations5
1
  • References9
  • Citations5
1

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Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity

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Visualization of kinase activity with FRET-based activity biosensors

Current Protocols in Molecular Biology

Jun 29, 2010

Charlene Depry, Jin Zhang

PMID: 20583095

DOI: 10.1002/0471142727.mb1815s91

Abstract

Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells...read more

Mentioned in this Paper

Biosensors
KARS gene
Immunofluorescence Microscopy
Biosensing Techniques
Phosphotransferases
Fluorescence Resonance Energy Transfer
Metazoa
Kinase Activity
Microscopy, Fluorescence

Trending Feeds

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

Methods in Molecular Biology

Using FRET-based reporters to visualize subcellular dynamics of protein kinase A activity

Methods in Molecular BiologyAugust 27, 2011
Charlene Depry, Jin Zhang
Methods in Molecular Biology

Imaging kinase activity at protein scaffolds

Methods in Molecular BiologySeptember 21, 2013
Maya T Kunkel, Alexandra C Newton
Paper Details
References
  • References9
  • Citations5
1
  • References9
  • Citations5
1
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