Visualization of molecular activities inside living cells with fluorescent labels

International Review of Cytology
Gertrude Bunt, Fred S Wouters

Abstract

The major task of modern cell biology is to identify the function and relation of the many different gene products, discovered by genomics and proteomics approaches, in the context of the living cell. To achieve this goal, an increasing toolbox of custom-designed biosensors based on fluorescent labels is available to study the molecular activities of the cellular machinery. An overview of the current status of the young field of molecular-cellular physiology is presented that includes the application of fluorescent labels in the design of biosensors and the major detection schemes used to extract their sensing information. In particular, the use of the photophysical phenomenon of Förster resonance energy transfer (FRET) as a powerful indicator of cellular biochemical events is discussed. In addition, we will point out the challenges and directions of the field and project the short-term future for the application of fluorescence-based biosensors in biology.

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Citations

Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sundar GanesanFred S Wouters
Apr 17, 2014·Assay and Drug Development Technologies·Thanh G NguyenRobert N Young
Aug 12, 2014·Biochemical Society Transactions·Kate M Fisher, Colin J Campbell
Sep 26, 2006·Drug Discovery Today·Steven A HaneyJing Zhang
Apr 5, 2017·Scientific Reports·Kazumasa MinamiHiroki Yokota
May 19, 2007·Molecular & Cellular Proteomics : MCP·Alessandro EspositoFred S Wouters
Apr 4, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hellen C Ishikawa-AnkerholdGregor P C Drummen
Jan 30, 2008·Current Protocols in Cell Biology·Alessandro Esposito, Fred S Wouters
Dec 4, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Siddhartha PalPabitra Chattopadhyay
Jul 11, 2006·Journal of Biomedical Optics·Alessandro EspositoFred S Wouters
Mar 1, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Johannes T WesselsFred S Wouters
Jun 22, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Johannes T WesselsFred S Wouters
Sep 16, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Quentin S HanleyThomas M Jovin
Sep 5, 2008·European Biophysics Journal : EBJ·Martin BeutlerRainer Heintzmann
Jan 7, 2014·Protoplasma·René EbrechtFred S Wouters
Feb 20, 2008·Nanotechnology·Teck Chuan LimTza-Huei Wang
Mar 7, 2020·Biomedical Optics Express·Alessandro Esposito
Jul 11, 2016·Analytical and Bioanalytical Chemistry·Antonio VarrialeSabato D'Auria
Feb 22, 2014·The Analyst·Siddhartha PalPabitra Chattopadhyay
Mar 19, 2021·BMC Plant Biology·Laura Gutiérrez-GarcíaAlbert Boronat
Jun 5, 2018·The Annals of Applied Statistics·Jan-Otto HooghoudtRasmus Waagepetersen

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