Visualization of Mouse Choroidal and Retinal Vasculature Using Fluorescent Tomato Lectin Perfusion

Translational Vision Science & Technology
Chunhua JiaoElliott H Sohn

Abstract

To develop a reliable and simplified method to assess choroid and retinal vasculature on whole mount and cross sections in mice using tomato lectin (TL; Lycopersicon esculentum). Albino mice (n = 27) received 1 mg/mL of TL (conjugated to Dylight-594) intravascularly through the tail vein, jugular vein, or cardiac left ventricle. Whole mounts of the retina and choroid were evaluated using fluorescence microscopy. Perfusion with GSL-IB4 conjugated to Dylight-594 and fluorescein isothiocyanate was performed to compare against labeling with TL. Co-labeling of choroidal endothelial cells with perfused TL on cross-sections with antibodies directed against the choriocapillaris-restricted endothelial cell marker CA4 was performed. The percentage of perfused choroidal and retinal vessels was assessed semiquantitatively. One mouse was subjected to thermal laser damage before perfusion to cause retinal and choroidal vasculature ablation. Intravascular injection of TL led to consistent, robust labeling of retinal and choroidal vascular walls. On cross-sections, choriocapillaris was co-labeled with CA4 and TL. On flat mount, TL perfusion resulted in better labeling of choroidal vessels using tail/jugular vein injection compared with cardiac...Continue Reading

References

Jan 1, 1987·Histochemistry·J AlroyE Skutelsky
Dec 1, 1996·The American Journal of Physiology·G ThurstonD M McDonald
Feb 1, 1997·Ophthalmology·R F MullinsG S Hageman
Nov 24, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R F Mullins, G S Hageman
Jul 15, 2000·Documenta Ophthalmologica. Advances in Ophthalmology·S A VinoresP A Campochiaro
Aug 30, 2008·Experimental Eye Research·Martin RudolfChristine A Curcio
Mar 7, 2009·Investigative Ophthalmology & Visual Science·Takayuki BabaGerard A Lutty
Oct 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Magali Saint-GeniezPatricia A D'Amore
Oct 21, 2010·Current Eye Research·Ryuhei HaraHidenobu Tanihara
Oct 16, 2012·Retina·Sato KurodaKohji Nishida
Nov 20, 2012·Biomedical Optics Express·Zhongwei ZhiRuikang K Wang
Jan 28, 2014·PloS One·Elliott H SohnRobert F Mullins
Feb 13, 2014·Investigative Ophthalmology & Visual Science·Elliott H SohnRobert F Mullins
Feb 22, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zsolt AblonczyAlexander G Marneros
Sep 16, 2014·American Journal of Ophthalmology·Richard F Spaide
Dec 9, 2014·Progress in Retinal and Eye Research·S Scott WhitmoreRobert F Mullins
Dec 24, 2014·Histochemistry and Cell Biology·Richard T RobertsonKenneth J Longmuir
Mar 6, 2015·Journal of Visualized Experiments : JoVE·Christopher C Rymer, Sunder Sims-Lucas
Apr 4, 2015·Current Eye Research·Sandra R MontezumaDeborah A Ferrington
May 30, 2015·Investigative Ophthalmology & Visual Science·Li ZhangMichael D Abràmoff
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elliott H SohnMichael David Abràmoff
Jul 29, 2016·Retinal Cases & Brief Reports·Elliott H SohnRobert F Mullins
Sep 30, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Trina ManaloPascal J Lafontant
Dec 10, 2016·Investigative Ophthalmology & Visual Science·Ipek OguzMilan Sonka
Jul 18, 2017·Investigative Ophthalmology & Visual Science·Richard F Spaide
Sep 8, 2018·Scientific Reports·Shemin ZengRobert F Mullins
Dec 26, 2018·Experimental Eye Research·Derrick J FeenstraAshwath Jayagopal
May 6, 2019·The American Journal of Pathology·Elliott H SohnRobert F Mullins

❮ Previous
Next ❯

Citations

Jul 13, 2021·Translational Vision Science & Technology·Nan ZhangJohn M Nickerson

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
fluorescence microscopy

Software Mentioned

GraphPad
Fiji
GraphPad prism

Related Concepts

Related Feeds

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.