PMID: 9555895Apr 29, 1998Paper

Visualization of penicillin-binding proteins during sporulation of Streptomyces griseus

Journal of Bacteriology
J Hao, K E Kendrick

Abstract

We used fluorescein-tagged beta-lactam antibiotics to visualize penicillin-binding proteins (PBPs) in sporulating cultures of Streptomyces griseus. Six PBPs were identified in membranes prepared from growing and sporulating cultures. The binding activity of an 85-kDa PBP increased fourfold by 10 to 12 h of sporulation, at which time the sporulation septa were formed. Cefoxitin inhibited the interaction of the fluorescein-tagged antibiotics with the 85-kDa PBP and also prevented septum formation during sporulation but not during vegetative growth. The 85-kDa PBP, which was the predominant PBP in membranes of cells that were undergoing septation, preferentially bound fluorescein-6-aminopenicillanic acid (Flu-APA). Fluorescence microscopy showed that the sporulation septa were specifically labeled by Flu-APA; this interaction was blocked by prior exposure of the cells to cefoxitin at a concentration that interfered with septation. We hypothesize that the 85-kDa PBP is involved in septum formation during sporulation of S. griseus.

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Citations

Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Dirk-Jan Scheffers, Mariana G Pinho
May 9, 2006·Molecular Microbiology·Rut Carballido-López
Feb 15, 2008·Antonie van Leeuwenhoek·Bjørn A Traag, Gilles P van Wezel
Feb 14, 2006·FEMS Microbiology Reviews·Antonio J Molina-HenaresJuan L Ramos
Nov 10, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Natsuko IchikawaNobuyuki Fujita
Oct 30, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexander Cambré, Abram Aertsen
May 1, 1999·Antimicrobial Agents and Chemotherapy·G ZhaoL C Blaszczak

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