Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysis

Current Protocols in Protein Science
Chang-Deng HuTom K Kerppola

Abstract

Protein interactions integrate stimuli from different signaling pathways and developmental programs. Bimolecular fluorescence complementation (BiFC) analysis has been developed for visualization of protein interactions in living cells. This approach is based on complementation between two fragments of a fluorescent protein when they are brought together by an interaction between proteins fused to the fragments, and it enables visualization of the subcellular locations of protein interactions in the normal cellular environment. It can be used for the analysis of many protein interactions and does not require information about the structures of the interaction partners. A multicolor BiFC approach has been developed for simultaneous visualization of interactions with multiple alternative partners in the same cell, based on complementation between fragments of engineered fluorescent proteins that produce bimolecular fluorescent complexes with distinct spectral characteristics. This enables comparison of subcellular distributions of different protein complexes in the same cell and allows analysis of competition between mutually exclusive interaction partners.

References

Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·N Johnsson, A Varshavsky
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·F RossiH M Blau
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·J N PelletierS W Michnick
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T NagaiA Miyawaki
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tom WehrmanHelen M Blau
Mar 11, 2003·Methods in Enzymology·Mark A HinkAntonie J W G Visser
Jun 18, 2003·The Biochemical Journal·Christopher D DeppmannElizabeth J Taparowsky
Jan 30, 2004·Molecular and Cellular Biology·Ingrid Remy, Stephen W Michnick
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Atsunari Tsuchisaka, Athanasios Theologis
Mar 30, 2004·Nature Cell Biology·Ingrid RemyStephen W Michnick
Apr 15, 2004·The Biochemical Journal·Gong-Hong WeiChih-Chuan Liang
Apr 20, 2004·Assay and Drug Development Technologies·Helen YuJohn K Westwick
May 4, 2004·Molecular and Cellular Biology·Asya V GrinbergTom K Kerppola
Jul 13, 2004·Current Opinion in Immunology·Tomasz Zal, Nicholas R J Gascoigne
Jul 30, 2004·The EMBO Journal·Oliver Rackham, Chris M Brown
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Keren Bracha-DroriNir Ohad
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
Jul 13, 2005·Chembiochem : a European Journal of Chemical Biology·Carsten SchultzTheodorus W J Gadella

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Citations

Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Peisu ZhangMark P Mattson
Dec 31, 2009·PLoS Pathogens·Xiaofei GaoPhilip R Hardwidge
Apr 2, 2011·PLoS Pathogens·Jennifer M BombergerBruce A Stanton
Nov 2, 2017·Current Protocols in Protein Science·Lisette G G C Verhoef, Mark Wade

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