PMID: 9185567Jul 1, 1997Paper

Visualization of RNA crystal growth by atomic force microscopy

Nucleic Acids Research
J D NgA McPherson

Abstract

The crystallization of transfer RNA (tRNA) was investigated using atomic force microscopy (AFM) over the temperature range from 4 to 16 degrees C, and this produced the first in situ AFM images of developing nucleic acid crystals. The growth of the (110) face of hexagonal yeast tRNAPhe crystals was observed to occur at steps on vicinal hillocks generated by multiple screw dislocation sources in the temperature range of 13.5-16 degrees C. Two-dimensional nucleation begins to dominate at 13.5 degrees C, with the appearance of three-dimensional nuclei at 12 degrees C. The changes in growth mechanisms are correlated with variations in supersaturation which is higher in the low temperature range. Growth of tRNA crystals was characterized by a strong anisotropy in the tangential step movement and transformation of growth modes on single crystals were directly observed by AFM over the narrow temperature range utilized. Finally, lattice resolution images of the molecular structure of surface layers were recorded. The implications of the strong temperature dependence of tRNAPhe crystal growth are discussed in view of improving and better controlling crystallization of nucleic acids.

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Citations

Sep 4, 2001·Chemistry : a European Journal·C M Niemeyer
Aug 24, 2006·Pharmaceutical Research·Xiangmin Liao, Timothy S Wiedmann
Nov 7, 2000·Colloids and Surfaces. B, Biointerfaces·Y G KuznetsovA McPherson
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Nov 6, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Alexander McPherson, Bob Cudney
Dec 2, 2010·HFSP Journal·Richard Giegé, Claude Sauter
Dec 3, 2013·Biochimica Et Biophysica Acta·Flavien PilletEtienne Dague
Apr 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Joseph D NgSijay Huang
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Jul 31, 2018·Reports on Progress in Physics·Isabel A OlsonMichael D Ward
Sep 3, 2015·NPJ Microgravity·Alexander McPherson, Lawrence James DeLucas
Jun 12, 2019·IUBMB Life·Catherine Florentz, Richard Giegé

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