PMID: 9554849May 9, 1998Paper

Visualization of single RNA transcripts in situ

Science
A M FeminoR H Singer

Abstract

Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes per molecule, and the light emitted by a single probe was calibrated. Points of light in exhaustively deconvolved images of hybridized cells gave fluorescent intensities and distances between probes consistent with single messenger RNA molecules. Analysis of beta-actin transcription sites after serum induction revealed synchronous and cyclical transcription from single genes. The rates of transcription initiation and termination and messenger RNA processing could be determined by positioning probes along the transcription unit. This approach extends the power of FISH to yield quantitative molecular information on a single cell.

References

Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P K ElderM J Getz

❮ Previous
Next ❯

Citations

May 29, 2000·Bulletin of Mathematical Biology·P SmolenJ H Byrne
Jul 13, 2002·Endocrine Pathology·Ernst J. M. Speel, Paul Komminoth
Jun 12, 2013·Histochemistry and Cell Biology·Sharon YungerYaron Shav-Tal
Feb 23, 2013·Pflügers Archiv : European journal of physiology·Zach Hensel, Jie Xiao
Jan 13, 2005·Development Genes and Evolution·Alexei Kurakin
Oct 19, 2007·Cardiovascular Toxicology·Tomasz Lipniacki, Marek Kimmel
May 21, 2005·DNA Repair·Hiroshi Kimura
Dec 4, 2003·Experimental Cell Research·Mei-Jun DuChih-Chuan Liang
Oct 12, 2001·Current Opinion in Neurobiology·J F GuzowskiP F Worley
Dec 14, 2002·Trends in Cell Biology·Jeffrey M Levsky, Robert H Singer
Aug 26, 1998·Trends in Cell Biology·R RizzutoR A Tuft
Jan 9, 2003·Trends in Biochemical Sciences·Ana Pombo
Jan 25, 2003·Methods : a Companion to Methods in Enzymology·Roeland W DirksHans J Tanke
Mar 10, 2007·Analytical Chemistry·Emily A SmithDanny L Brower
Feb 1, 2012·Analytical Chemistry·Andrew E PrigodichChad A Mirkin
Sep 18, 2012·Analytical Chemistry·Suxian HuangYong Chen
Apr 13, 2013·Analytical Chemistry·Douglas P ShepherdJames H Werner
May 24, 2013·Chemical Reviews·Haifeng DongXueji Zhang
Sep 24, 2013·Chemical Reviews·Jens Michaelis, Barbara Treutlein
Nov 24, 2007·Journal of the American Chemical Society·Dwight S SeferosChad A Mirkin
Nov 30, 2013·Journal of the American Chemical Society·Kyu Young HanTaekjip Ha
May 22, 2013·Journal of Medicinal Chemistry·Meirav SegalBilha Fischer
Nov 6, 2009·European Journal of Human Genetics : EJHG·Peter S MasnySara T Winokur
Jul 22, 2011·Nature·Gene-Wei Li, X Sunney Xie
Nov 3, 2010·Nature Biotechnology·Harry M T ChoiNiles A Pierce
Jun 28, 2011·Nature Cell Biology·Daan NoordermeerWouter de Laat
Nov 29, 2011·Nature Cell Biology·Shalev ItzkovitzAlexander van Oudenaarden
Sep 14, 2004·Nature Genetics·Cameron S OsbornePeter Fraser
Sep 23, 2008·Nature Methods·Arjun RajSanjay Tyagi
Apr 8, 2009·Nature Methods·Philip J SantangeloJames E Crowe
Apr 13, 2010·Nature Methods·Chatarina LarssonMats Nilsson
Jul 20, 2010·Nature Methods·Sharon YungerYaron Shav-Tal
Jan 18, 2011·Nature Methods·Timothée LionnetRobert H Singer
Apr 1, 2011·Nature Methods·Fuchou TangM Azim Surani
Apr 6, 2011·Nature Methods·Shalev Itzkovitz, Alexander van Oudenaarden
Feb 19, 2013·Nature Methods·Marshall J Levesque, Arjun Raj
Aug 6, 2013·Nature Methods·Marshall J LevesqueArjun Raj
Aug 13, 2013·Nature Methods·Clinton H Hansen, Alexander van Oudenaarden
Aug 25, 2005·Nature Methods·Paola CapodieciRobert H Singer
Feb 4, 2012·Nature Protocols·Tatjana TrcekRobert H Singer
Aug 21, 2013·Nature Protocols·Anna LyubimovaAlexander van Oudenaarden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.