Visualization of the turkey erythrocyte beta-adrenergic receptor

Journal of Supramolecular Structure
O Durieu-TrautmannA D Strosberg

Abstract

We have recently described the affinity chromatography purification of the turkey erythrocyte beta-adrenergic receptor. The minute amounts obtained initially precluded extensive biochemical characterization. To improve the yield of the receptor, the erythrocyte membranes have been prepared by a new method. This procedure resulted in a 10-fold higher receptor density in comparison with the membrane preparation used previously. The new membranes also contained a catecholamine-sensitive guanine triphosphatase and an adenylate cyclase sensitive to Gpp(NH)p and l-epinephrine. Solubilization by a double digitonin extraction resulted in a preparation containing 4-6 pmoles of 3H-dihydroalprenolol binding sites per mg of membrane protein. A single step of affinity chromatography on alprenolol-sepharose of the soluble digitonin extract resulted in an additional 1,000-fold purification of the receptor. The overall purification factor was 20,000 relative to the binding activity of the crude membrane preparations. Electrophoresis is SDS-polyacrylamide of iodinated purified beta-receptors revealed, after autoradiography, the presence of four major components. Three of these, corresponding to molecular weights of 170,000, 33,000, and 30,000, ...Continue Reading

References

Feb 28, 1979·Biochemical and Biophysical Research Communications·S BottariA D Strosberg
Dec 8, 1976·Biochimica Et Biophysica Acta·D Cassel, Z Selinger
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G VauquelinA D Strosberg
Mar 23, 1978·Nature·D Atlas, A Levitzki
Aug 1, 1979·European Journal of Biochemistry·G VauquelinA D Strosberg
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S JacobsP Cuatrecasas
Jun 15, 1976·Biochemistry·A B Caldwell
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R W AlexanderR J Lefkowitz
Mar 1, 1976·European Journal of Pharmacology·K N SalmanP N Patil
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A LevitzkiM L Steer
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J PithaS P Baker
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J G GuilletA D Strosberg
Jan 1, 1985·Preparative Biochemistry·S T George, C C Malbon
Jan 1, 1987·Journal of Receptor Research·M P ChapotA D Strosberg
Jan 1, 1983·Journal of Cellular Biochemistry·P O CouraudA D Strosberg

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