PMID: 8605226Feb 9, 1996Paper

Visualization of yellowish-orange luminescence from cuprous metallothioneins in liver of Long-Evans Cinnamon rat

Biochimica Et Biophysica Acta
K NakayamaN Kasai

Abstract

We describe the first use of an emission probe, based on the cuprous thiolate chromophore, for direct microscopical observation of cuprous metallothioneins located in liver of 15-week-old (just before spontaneous hepatitis) Long-Evans Cinnamon rats. The rats show remarkable accumulations of copper and cuprous metallothioneins. In the mildly fixed liver, we visualized the same yellowish-orange luminescence as the specific emission from cuprous metallothioneins, following excitation in 330-385 nm region. In liver from Long-Evans Agouti rat, a counter part of Long-Evans Cinnamon rat, no similar luminescence was found. So, it was thought that cuprous metallothioneins accumulated in the Long-Evans Cinnamon rat liver might emit the yellowish-orange light. To verify this presumption, we tentatively defined three histochemical criteria, quenching tests by oxidation, protonation and mercury treatment, based on the coordination chemical characteristics of metallothioneins. The emission completely satisfied these criteria. Furthermore, the reliability of these criteria was supported by immunocytochemical and biochemical results. Consequently, all results sufficiently indicate that the yellowish-orange luminescence in the Long-Evans Cinnam...Continue Reading

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Citations

Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Suzuki-KurasakiM Kurasaki
Feb 14, 2007·Journal of Gastroenterology and Hepatology·Akiko SaitoHiroshi Hara
May 12, 1998·The American Journal of Physiology·M KurasakiM Suzuki-Kurasaki
Feb 24, 1997·Biochemical and Biophysical Research Communications·M NakamuraT Ohyama
May 24, 2001·Archives of Biochemistry and Biophysics·N ShishidoM Nakamura

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