Visualizing Bdellovibrio bacteriovorus by Using the tdTomato Fluorescent Protein

Applied and Environmental Microbiology
Somdatta MukherjeeDaniel E Kadouri

Abstract

Bdellovibrio bacteriovorus is a Gram-negative bacterium that belongs to the delta subgroup of proteobacteria and is characterized by a predatory life cycle. In recent years, work has highlighted the potential use of this predator to control bacteria and biofilms. Traditionally, the reduction in prey cells was used to monitor predation dynamics. In this study, we introduced pMQ414, a plasmid that expresses the tdTomato fluorescent reporter protein, into a host-independent strain and a host-dependent strain of B. bacteriovorus 109J. The new construct was used to conveniently monitor predator proliferation in real time, in different growth conditions, in the presence of lytic enzymes, and on several prey bacteria, replicating previous studies that used plaque analysis to quantify B. bacteriovorus. The new fluorescent plasmid also enabled us to visualize the predator in liquid cultures, in the context of a biofilm, and in association with human epithelial cells.

References

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Citations

Aug 17, 2016·Scientific Reports·Eric G RomanowskiRobert M Q Shanks
Sep 1, 2016·PloS One·Shilpi GuptaDaniel E Kadouri
Jan 19, 2020·Applied and Environmental Microbiology·Daniel YoudkesE Jurkevitch
May 29, 2019·Environmental Microbiology·Rajesh SathyamoorthyE Jurkevitch
May 1, 2020·Frontiers in Microbiology·Eleni BratanisEwa Bukowska-Faniband
Apr 8, 2021·Current Biology : CB·Benjamin R WucherCarey D Nadell
Mar 19, 2021·FEMS Microbiology Ecology·Rajesh SathyamoorthyE Jurkevitch

Related Concepts

Bdellovibrio
Epithelial Cells
Luminescent Proteins
Episomes
Staining and Labeling
Gene Expression
Genes, Reporter
Bacterial Count Measurement
Gram-Negative Bacteria
Plasmids

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