Visualizing cellular phosphoinositide pools with GFP-fused protein-modules

Science's STKE : Signal Transduction Knowledge Environment
Tamas Balla, Péter Várnai

Abstract

Inositol phospholipids are well known for their pivotal role in calcium signaling as precursors of important second messengers generated in response to various stimuli. However, over the last 10 years, inositides have also emerged as universal signaling components present in virtually every membrane of eukaryotic cells. These lipids are locally produced and degraded by the numerous inositide kinase and phosphatase enzymes, to control the recruitment and activity of protein signaling complexes in specific membrane compartments. The spatial and temporal constraints imposed on changes in cellular inositides pose new challenges in finding experimental techniques through which such changes can be examined. Taking advantage of the protein domains selected by evolution to recognize cellular phosphoinositides, we have created fluorescent molecules by fusing these domains to the improved version of green fluorescent protein (EGFP); the distribution of these fusion proteins can be followed within live cells, thereby reporting on changes in phosphoinositides. Although this technique is one of the few that provide information on phosphoinositide dynamics in live cells with subcellular resolution and has rapidly gained popularity, it also h...Continue Reading

References

Sep 27, 1996·The Journal of Biological Chemistry·H StenmarkA D'Arrigo
Nov 1, 1996·Protein Science : a Publication of the Protein Society·C P Ponting
Jan 19, 1999·Methods in Cell Biology·J Lippincott-SchwartzJ Ellenberg
Mar 24, 1999·Trends in Cell Biology·J EllenbergJ F Presley
Jul 8, 1999·Molecular Cell·T G KutateladzeM Overduin
Dec 11, 1999·Nucleic Acids Research·A BatemanE L Sonnhammer
Jun 29, 2000·Trends in Pharmacological Sciences·T BallaP Várnai
Sep 6, 2000·The EMBO Journal·D J GilloolyH Stenmark
Feb 15, 2001·Nature Biotechnology·A G HarpurP I Bastiaens
Jul 4, 2001·Nature Cell Biology·F KanaiM B Yaffe

❮ Previous
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Citations

Jul 27, 2011·Analytical and Bioanalytical Chemistry·Weigang HuangQisheng Zhang
May 24, 2007·Pflügers Archiv : European journal of physiology·Yuntao S Mao, Helen L Yin
Nov 13, 2003·Current Opinion in Plant Biology·David Ehrhardt
Sep 4, 2009·Journal of the American Chemical Society·Shuhei HashiroTeruyuki Nagamune
Mar 24, 2006·Nature Methods·Tor Erik Rusten, Harald Stenmark
Jul 30, 2005·Nature Reviews. Drug Discovery·Markus R Wenk
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Matthias P Wymann, Roger Schneiter
Mar 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Liang-Wei GongDerek Toomre
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Pingbo ZhangMiho Iijima
May 29, 2013·The Journal of General Physiology·Radda RusinovaOlaf S Andersen
Sep 25, 2003·Annual Review of Plant Biology·Harold J G Meijer, Teun Munnik
Sep 10, 2013·Journal of Negative Results in Biomedicine·Jaeda C Coutinho-BuddMark J Zylka
Feb 27, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Moshe ParnasBaruch Minke
Mar 8, 2006·Nature Chemical Biology·Cristian G BologaEric R Prossnitz
May 12, 2009·Nature Chemical Biology·Megan K DennisEric R Prossnitz
May 14, 2004·Nature·Toshiki Itoh, Pietro De Camilli
Jul 25, 2006·FEBS Letters·Markus R Wenk
Dec 10, 2009·The Journal of Cell Biology·Gordon PolevoyJulie A Brill
Apr 29, 2016·Inside the Cell·Ashley A GeorgeSusan E Brockerhoff
May 8, 2009·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Dimitar P ZankovMinoru Horie
Jan 8, 2010·The Journal of Investigative Dermatology·Damien CalayRobert Gniadecki
Jun 22, 2005·Biology of the Cell·Guillaume Halet
Mar 26, 2016·Analytical Chemistry·Edwin ArauzJie Chen
Jul 11, 2006·Methods : a Companion to Methods in Enzymology·Kartik Narayan, Mark A Lemmon
Jan 9, 2003·Annual Review of Physiology·Michael P Czech

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