Visualizing cold spots: TRPM8-expressing sensory neurons and their projections

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Ajay DhakaArdem Patapoutian

Abstract

Environmental stimuli such as temperature and pressure are sensed by dorsal root ganglion (DRG) neurons. DRG neurons are heterogeneous, but molecular markers that identify unique functional subpopulations are mainly lacking. ThermoTRPs are members of the transient receptor potential family of ion channels and are gated by shifts in temperature. TRPM8 is activated by cooling, and TRPM8-deficient mice have severe deficits in cool thermosensation. The anatomical and functional properties of TRPM8-expressing fibers have not been not comprehensively investigated. We use mice engineered to express the farnesylated enhanced green fluorescent protein (EGFPf) from the TRPM8 locus (TRPM8(EGFPf)) to explore this issue. Virtually all EGFPf-positive cultured DRG neurons from hemizygous mice (TRPM8(EGFPf/+)) responded to cold and menthol. In contrast, EGFPf-positive DRGs from homozygous mice (TRPM8(EGFPf/EGFPf)) had drastically reduced cold responses and no menthol responses. In vivo, EGFPf-positive neurons marked a unique population of DRG neurons, a majority of which do not coexpress nociceptive markers. The fraction of DRG neurons expressing EGFPf was not altered under an inflammatory condition, although an increase in TRPV1-coexpressing ...Continue Reading

Citations

Apr 23, 2008·Neurochemical Research·Jen-Kun Cheng, Ru-Rong Ji
Nov 6, 2010·Archives of Pharmacal Research·Eduardo Gascon, Aziz Moqrich
Jan 19, 2011·ACS Chemical Neuroscience·Félix Viana
Jan 1, 2009·Nature Reviews. Drug Discovery·Ardem PatapoutianClifford J Woolf
Nov 12, 2010·Nature Reviews. Neuroscience·Andrew J Todd
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yuji KarashimaThomas Voets
Aug 31, 2011·The Journal of General Physiology·Diana M Bautista, Ellen A Lumpkin
May 31, 2008·British Journal of Anaesthesia·A Dray
Oct 9, 2013·Annual Review of Cell and Developmental Biology·David Julius
Mar 18, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daniel D McCoyDavid D McKemy
Sep 7, 2012·Investigative Ophthalmology & Visual Science·Ashlee RobbinsIan D Meng
Nov 3, 2010·The Journal of Clinical Investigation·Adrienne E Dubin, Ardem Patapoutian
Nov 3, 2010·The Journal of Clinical Investigation·Qiufu Ma
Apr 19, 2008·Molecular Pain·Carlos Belmonte, Félix Viana
Aug 5, 2009·Neural Development·Robert GasperiniLisa Foa
Dec 23, 2011·Development·Y Albert PanAlexander F Schier
Oct 9, 2009·PloS One·Ombretta CaspaniPaul A Heppenstall
Mar 17, 2011·PloS One·Koji TajinoShigeo Kobayashi
Jan 7, 2014·PloS One·Marina HovakimyanMartin Witt
Feb 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wendy M KnowltonDavid D McKemy
Mar 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Judith P GoldenSanjay Jain
May 30, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rainer H Straub
Sep 27, 2014·Membranes·Clotilde Ferrandiz-HuertasAntonio Ferrer-Montiel
Apr 21, 2012·Trends in Neurosciences·Francois Lallemend, Patrik Ernfors
Jan 8, 2016·Molecular Neurobiology·Changbin KeYuke Tian
Oct 5, 2010·Current Opinion in Neurobiology·Yang Liu, Qiufu Ma
Oct 24, 2009·Gene Expression Patterns : GEP·Susanne StaafPatrik Ernfors
Oct 14, 2009·Neuroscience and Biobehavioral Reviews·Raf J Schepers, Matthias Ringkamp
May 5, 2009·The Journal of Pain : Official Journal of the American Pain Society·Heather L RossiJohn K Neubert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.