Visualizing cytoskeleton dynamics in mammalian cells using a humanized variant of monomeric red fluorescent protein

FEBS Letters
Markus FischerAnnette Müller-Taubenberger

Abstract

Fluorescent proteins are versatile tools for live cell imaging studies. In particular, recent progress was achieved in the development of monomeric red fluorescent proteins (mRFPs) that show improved properties in respect to maturation and intracellular fluorescence. mRFPmars, a red fluorescent protein designed especially for the use in Dictyostelium, proved to be a brilliant label for different cytoskeletal elements. Here we report on the synthesis of a humanized version of a monomeric RFP, mRFPruby, which differs in sequence from mRFPmars in four amino acids and has a codon usage that is optimized for the application in mammalian cells. In order to demonstrate the usefulness of this new mRFP variant, mRFPruby fused to beta-actin was expressed in different mouse cell lines and used to visualize actin cytoskeleton dynamics by live cell microscopy.

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Citations

Aug 21, 2007·Applied Microbiology and Biotechnology·Annette Müller-Taubenberger, Kurt I Anderson
Jun 10, 2008·Nature Methods·Julia RiedlRoland Wedlich-Soldner
Mar 3, 2010·Nature Methods·Julia RiedlRoland Wedlich-Söldner
Jun 4, 2011·Science·Julia Domínguez-EscobarRut Carballido-López
Dec 7, 2006·PLoS Biology·María A RujanoHarm H Kampinga
Apr 30, 2013·Development, Growth & Differentiation·Takaya Abe, Toshihiko Fujimori
Jun 23, 2006·European Journal of Cell Biology·Annette Müller-TaubenbergerKlemens Rottner
Nov 26, 2010·Seminars in Cell & Developmental Biology·Marthe H R LudtmannRobin S B Williams
May 26, 2009·Biochimica Et Biophysica Acta·Michel J VosHarm H Kampinga
Sep 19, 2007·The Journal of Biological Chemistry·Jurre HagemanHarm H Kampinga
Aug 19, 2007·Development·Christopher M WestZhuo A Wang
Apr 14, 2011·Journal of Cell Science·Jerry H YuRoland Wedlich-Söldner

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