Visualizing dopamine released from living cells using a nanoplasmonic probe

Nanoscale
W W QinDi Li

Abstract

We report the development of an ultrasensitive nanoplasmonic probe for discriminative detection and imaging of dopamine released from living cells. The sensing mechanism is based on the dopamine-induced seeded-growth of Au nanoparticles (Au NPs) that leads to the shift of the plasmon band. This platform allows for the detection of dopamine with a detection limit down to 0.25 pM within 1 min. This nanoplasmonic assay is further applied to visualize the release of dopamine from living rat pheochromocytoma (PC12) cells under ATP-stimulation with dark-field microscopy (DFM). The DFM results together with real time fluorescence imaging of PC12 cells stained with the Fluo calcium indicator, suggested that ATP stimulated-release of dopamine is concomitant with the Ca(2+) influx, and the influx of Ca(2+) is through ATP-activated channels instead of the voltage-gated Ca(2+) channel (VGC).

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Citations

Feb 9, 2016·The Analyst·Zhiqin YuanChao Lu
Feb 6, 2017·Frontiers in Pharmacology·Weiwei QinYuanqing Zhang
Jun 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Faten Bashar Kamal Eddin, Yap Wing Fen
Feb 23, 2020·Sensors·Faten Bashar Kamal Eddin, Yap Wing Fen
Nov 22, 2019·Analytical Chemistry·Zhongju YeLehui Xiao

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