Visualizing enveloping layer glycans during zebrafish early embryogenesis

Proceedings of the National Academy of Sciences of the United States of America
Jeremy M BaskinCarolyn R Bertozzi

Abstract

Developmental events can be monitored at the cellular and molecular levels by using noninvasive imaging techniques. Among the biomolecules that might be targeted for imaging analysis, glycans occupy a privileged position by virtue of their primary location on the cell surface. We previously described a chemical method to image glycans during zebrafish larval development; however, we were unable to detect glycans during the first 24 hours of embryogenesis, a very dynamic period in development. Here we report an approach to the imaging of glycans that enables their visualization in the enveloping layer during the early stages of zebrafish embryogenesis. We microinjected embryos with azidosugars at the one-cell stage, allowed the zebrafish to develop, and detected the metabolically labeled glycans with copper-free click chemistry. Mucin-type O-glycans could be imaged as early as 7 hours postfertilization, during the gastrula stage of development. Additionally, we used a nonmetabolic approach to label sialylated glycans with an independent chemistry, enabling the simultaneous imaging of these two distinct classes of glycans. Imaging analysis of glycan trafficking revealed dramatic reorganization of glycans on the second time scale,...Continue Reading

References

May 29, 2012·Glycoconjugate Journal·Heather R Flanagan-Steet, Richard Steet
Jul 14, 1987·Biochimica Et Biophysica Acta·K BuckinghamM C Ganoza
Feb 22, 2013·Bioconjugate Chemistry·Mohammad RashidianMark D Distefano
Sep 14, 2012·Biomacromolecules·Gregory N GroverHeather D Maynard
Mar 28, 2013·Chemical Reviews·Prakasam ThirumuruganKrzysztof Jozwiak
Nov 11, 2010·Journal of the American Chemical Society·David Soriano del AmoPeng Wu
Apr 26, 2011·Journal of the American Chemical Society·Josep RayoMichael M Meijler
Mar 23, 2012·Journal of the American Chemical Society·Mohammad RashidianMark D Distefano
Aug 25, 2012·Journal of the American Chemical Society·Rajesh Chakrabarty, Peter J Stang
Apr 20, 2011·Chemical Communications : Chem Comm·Chung-Yi Wu, Chi-Huey Wong
Jun 22, 2011·Molecular BioSystems·Kimberly E Beatty
Jul 18, 2012·Chemical Communications : Chem Comm·Pamela V Chang, Carolyn R Bertozzi
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Richard W GanttJon S Thorson
Jun 16, 2011·Journal of Visualized Experiments : JoVE·Hao JiangPeng Wu
Jan 21, 2014·ACS Chemical Biology·David M PattersonJennifer A Prescher
Jun 21, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiu Zhang, Yan Zhang
Mar 5, 2016·Cell Chemical Biology·Kelly N ChuhMatthew R Pratt
May 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Rosemary M OnjikoPeter Nemes
Nov 15, 2011·Current Opinion in Chemical Biology·Yingke XuDerek K Toomre
Sep 13, 2015·Analytical and Bioanalytical Chemistry·Edward BodnarHélène Perreault
Jul 5, 2012·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Md Monsur AliWeian Zhao
Nov 28, 2012·Current Opinion in Chemical Biology·Koichi Fukase, Katsunori Tanaka
Apr 23, 2015·Journal of the American Chemical Society·Peyton ShiehCarolyn R Bertozzi
Dec 25, 2016·Histochemistry and Cell Biology·Anthony Corfield
Mar 5, 2014·Organic Letters·Ellen M SlettenCarolyn R Bertozzi
Jan 29, 2017·Chembiochem : a European Journal of Chemical Biology·Alexander M XuNicholas A Melosh
Feb 14, 2015·Angewandte Chemie·Christopher WitteChristian P R Hackenberger
Jun 12, 2012·Angewandte Chemie·Shenliang WangEric T Kool
Aug 1, 2015·Angewandte Chemie·Paresh AgarwalCarolyn R Bertozzi
Jun 21, 2016·Nature Communications·Samira Husen AlamudiYoung-Tae Chang
Jul 27, 2010·Organic & Biomolecular Chemistry·Isao KiiTakamitsu Hosoya
Mar 27, 2019·Glycobiology·Sam J MoonsChristian Büll
May 10, 2019·Chemical Communications : Chem Comm·Xiaohong WenKemin Wang

Citations

Mar 1, 1985·Developmental Biology·C B Kimmel, R D Law
Mar 17, 2000·Science·Eliana Saxon, Carolyn R Bertozzi
Sep 19, 2001·Accounts of Chemical Research·H C Hang, Carolyn R Bertozzi
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·C B Shuster, D R Burgess
Dec 12, 2002·Journal of the American Chemical Society·Eliana SaxonCarolyn R Bertozzi
Jan 31, 2003·Biotechnology and Bioengineering·P A De BankK M Shakesheff
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Howard C HangCarolyn R Bertozzi
Jun 11, 2004·Annual Review of Biochemistry·Robert S Haltiwanger, John B Lowe
Jun 29, 2004·Molecular Immunology·Clifford A Lowell
Sep 24, 2004·Biochemistry·Sarah J LuchanskyCarolyn R Bertozzi
Feb 8, 2005·Trends in Cell Biology·Roger AlbertsonWilliam Sullivan
Dec 3, 2005·Glycobiology·Yann GuérardelKay-Hooi Khoo
Jan 13, 2006·Trends in Cell Biology·Dimitris Beis, Didier Y R Stainier
Jan 13, 2006·Nature Chemical Biology·Jennifer A Prescher, Carolyn R Bertozzi
Apr 15, 2006·Science·Ben N G GiepmansRoger Y Tsien
May 30, 2006·Nature Medicine·Karina YanivBrant M Weinstein
Sep 9, 2006·Cell·Jennifer A Prescher, Carolyn R Bertozzi
Oct 20, 2006·Angewandte Chemie·Anouk DirksenPhilip E Dawson
Jun 21, 2007·Journal of the American Chemical Society·Pamela V ChangCarolyn R Bertozzi
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeremy M BaskinCarolyn R Bertozzi
May 3, 2008·Science·Scott T LaughlinCarolyn R Bertozzi
Jul 9, 2008·Trends in Molecular Medicine·Ajit Varki
Aug 16, 2008·Glycoconjugate Journal·Lan-Yi ChangYann Guérardel
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Scott T Laughlin, Carolyn R Bertozzi
Feb 24, 2009·Nature Methods·Ying ZengJames C Paulson
Apr 21, 2009·Methods in Molecular Biology·Sean G Megason

Related Concepts

Embryo
Microinjections
Mucin-1 protein
Larval Development
Trafficking
Embryonic Development
Imaging Techniques
Cell Division Phases
Zebrafish
Gastrula

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