Fluorescence Ca(2+) imaging enables large-scale recordings of neural activity, but collective dynamics across mammalian brain regions are generally inaccessible within single fields of view. Here we introduce a two-photon microscope possessing two articulated arms that can simultaneously image two brain areas (∼0.38 mm(2) each), either nearby or distal, using microendoscopes. Concurrent Ca(2+) imaging of ∼100-300 neurons in primary visual cortex (V1) and lateromedial (LM) visual area in behaving mice revealed that the variability in LM neurons' visual responses was strongly dependent on that in V1, suggesting that fluctuations in sensory responses propagate through extended cortical networks.
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror
Network analysis of corticocortical connections reveals ventral and dorsal processing streams in mouse visual cortex
Long-Term Two-Photon Calcium Imaging of Neuronal Populations with Subcellular Resolution in Adult Non-human Primates
Microelectronics, bioinformatics and neurocomputation for massive neuronal recordings in brain circuits with large scale multielectrode array probes
Spherical statistics for characterizing the spatial distribution of deep brain stimulation effects on neuronal activity
Modeling of Cerebral Oxygen Transport Based on In vivo Microscopic Imaging of Microvascular Network Structure, Blood Flow, and Oxygenation
Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
Influence of the oxazole ring connection on the fluorescence of oxazoyl-triphenylamine biphotonic DNA probes
Two-photon calcium imaging of the medial prefrontal cortex and hippocampus without cortical invasion
Local synaptic inputs support opposing, network-specific odor representations in a widely projecting modulatory neuron
Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions.
State-of-the-Art Techniques to Causally Link Neural Plasticity to Functional Recovery in Experimental Stroke Research
In vivo wide-field calcium imaging of mouse thalamocortical synapses with an 8 K ultra-high-definition camera
Multichannel fiber photometry for mapping axonal terminal activity in a restricted brain region in freely moving mice
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
In vivo two-photon imaging of mouse hippocampal neurons in dentate gyrus using a light source based on a high-peak power gain-switched laser diode
An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator.
Measurement and visualization of stimulus-evoked tissue dynamics in mouse barrel cortex using phase-sensitive optical coherence tomography
BRAIN Initiative Cell Census Network (BICCN)
The BRAIN Initiative Cell Census Network aims to identify and provide experimental access to the different brain cell types to determine their roles in health and disease. Discover the latest research from researchers in the BRAIN Initiative Cell Census Network here.