Visualizing microtubule structural transitions and interactions with associated proteins

Current Opinion in Structural Biology
E Nogales, Rui Zhang

Abstract

Microtubules (MTs) have been the subject of cryo-electron microscopy (cryo-EM) studies since the birth of this technique. Although MTs pose some unique challenges, having to do with the presence of a MT seam, lattice variability and disorder, MT cryo-EM reconstructions are steadily improving in resolution and providing exciting new insights into MT structure and function. Recent work has lead to the atomic-detail visualization of lateral contacts between tubulin subunits and the conformational changes that give rise to strain in the MT lattice accompanying GTP hydrolysis. Cryo-EM has also been invaluable in describing the interactions between MTs and MT associated proteins (MAPs), which function to regulate MT dynamic instability, move cargoes, or contribute to other MT cellular processes.

References

Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D D Hackney
Mar 1, 1986·The Journal of Cell Biology·E M MandelkowE Mandelkow
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Jun 17, 1998·Nature Structural Biology·E NogalesJ Löwe
Feb 16, 1999·Cell·E NogalesK H Downing
Oct 16, 2002·Structure·Huilin LiKenneth H Downing
Jul 15, 2003·The Journal of Biological Chemistry·Ikuko Hayashi, Mitsuhiko Ikura
Apr 14, 2005·The Journal of Biological Chemistry·I R GibbonsAndrew P Carter
May 2, 2007·The Journal of Cell Biology·Charles V Sindelar, Kenneth H Downing
Sep 25, 2007·Molecular Cell·Kevin C Slep, Ronald D Vale
Mar 7, 2008·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Jan 31, 2009·Current Opinion in Cell Biology·Arne Gennerich, Ronald D Vale
Jul 14, 2010·Current Biology : CB·Niels Galjart
Oct 5, 2010·Nature Reviews. Drug Discovery·Charles Dumontet, Mary Ann Jordan
Oct 27, 2010·The Journal of Cell Biology·Franck J FourniolCarolyn A Moores
Sep 22, 2012·Science·W B RedwineA E Leschziner
Oct 23, 2012·Nature Structural & Molecular Biology·Gregory M AlushinEva Nogales
Jan 5, 2013·Science·Andrea E ProtaMichel O Steinmetz
Sep 17, 2013·Structure·Yifan SongDavid Baker
Sep 26, 2013·Nature Reviews. Molecular Cell Biology·Anthony J RobertsStan A Burgess
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Adeline GouletCarolyn A Moores
Feb 11, 2014·Current Biology : CB·Sebastian P MaurerThomas Surrey
Mar 19, 2014·Nature Cell Biology·Minhajuddin SirajuddinRonald D Vale
Aug 20, 2014·The Journal of Cell Biology·Carsten Janke
Nov 12, 2014·The Journal of Cell Biology·Gary J Brouhard, Luke M Rice
Mar 10, 2015·Nature Structural & Molecular Biology·Saikat ChowdhuryGabriel C Lander
Mar 31, 2015·Science·Linas UrnaviciusAndrew P Carter
May 12, 2015·Cell·Christopher P GarnhamAntonina Roll-Mecak
Oct 2, 2015·Journal of Structural Biology·Rui Zhang, Eva Nogales

❮ Previous
Next ❯

Citations

Aug 20, 2016·ACS Chemical Biology·Kathryn P WallLoren E Hough
Mar 17, 2017·Molecular Biology of the Cell·Lauren D'AngeloKenneth A Myers
Mar 23, 2017·Chemical Society Reviews·H Hess, Jennifer L Ross
Mar 31, 2017·Scientific Reports·Lurlène AkendengueAnne Marie Wehenkel
Feb 24, 2017·Scientific Reports·Maria E Fuentes-PérezMaria A Oliva
Oct 11, 2017·Nature Structural & Molecular Biology·Joseph AthertonAnna Akhmanova
Nov 10, 2017·ELife·Rui ZhangEva Nogales
Feb 11, 2018·Scientific Reports·Davide ZabeoJohanna L Höög
Aug 24, 2017·Nature Reviews. Molecular Cell Biology·Johanna Roostalu, Thomas Surrey
May 22, 2019·American Journal of Physiology. Endocrinology and Metabolism·Ming YanC Yan Cheng
Nov 5, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Paul Hendrik SchummelRoland Winter
Jun 7, 2018·Acta Crystallographica. Section D, Structural Biology·Joseph AthertonCarolyn A Moores
Aug 9, 2019·Annual Review of Cell and Developmental Biology·Mithila Burute, Lukas C Kapitein
Jul 25, 2019·Physical Review. E·Arshia ZarrinAidan I Brown
Aug 6, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth H KelloggEva Nogales
Nov 26, 2020·Biology of Reproduction·Naina Kumar, Amit Kant Singh
Dec 7, 2020·Drug Discovery Today·Zlata Boiarska, Daniele Passarella
Aug 9, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Arun Kanakkanthara, John H Miller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biophysics of CRISPR

This feed focuses on broad characteristics of the CRISPR system and the proteins associated with it.