Visualizing peripheral nerve regeneration by whole mount staining

PloS One
Xin-peng Dun, David B Parkinson

Abstract

Peripheral nerve trauma triggers a well characterised sequence of events both proximal and distal to the site of injury. Axons distal to the injury degenerate, Schwann cells convert to a repair supportive phenotype and macrophages enter the nerve to clear myelin and axonal debris. Following these events, axons must regrow through the distal part of the nerve, re-innervate and finally are re-myelinated by Schwann cells. For nerve crush injuries (axonotmesis), in which the integrity of the nerve is maintained, repair may be relatively effective whereas for nerve transection (neurotmesis) repair will likely be very poor as few axons may be able to cross between the two parts of the severed nerve, across the newly generated nerve bridge, to enter the distal stump and regenerate. Analysing axon growth and the cell-cell interactions that occur following both nerve crush and cut injuries has largely been carried out by staining sections of nerve tissue, but this has the obvious disadvantage that it is not possible to follow the paths of regenerating axons in three dimensions within the nerve trunk or nerve bridge. To try and solve this problem, we describe the development and use of a novel whole mount staining protocol that allows th...Continue Reading

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Citations

Feb 1, 2017·The Journal of Cell Biology·Thomas MindosDavid B Parkinson
Dec 16, 2016·Journal of Neurochemistry·Sheridan L RobertsDavid B Parkinson
Mar 24, 2018·Biofabrication·Eugen B PetcuPaul D Dalton
Aug 6, 2017·The AAPS Journal·Jelena M Janjic, Vijay S Gorantla
Nov 15, 2019·Annals of Clinical and Translational Neurology·Giulia ZanettiCesare Montecucco
Oct 16, 2019·Nanomedicine·Igor D LuzhanskyMikhail Y Berezin
Mar 2, 2017·International Journal of Molecular Sciences·Xin-Peng Dun, David B Parkinson
Jan 11, 2020·Frontiers in Molecular Neuroscience·Bing ChenXin-Peng Dun
Aug 28, 2020·Frontiers in Cellular Neuroscience·Bing ChenXin-Peng Dun
Oct 27, 2020·Facial Plastic Surgery & Aesthetic Medicine·Pin-Keng ShihShih-Chi Wu
Nov 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavia MillesiChristine Radtke
Jul 3, 2017·Journal of Neuroscience Methods·Simone GrossKaspar Matiasek
Sep 14, 2021·ACS Biomaterials Science & Engineering·Riley A SuharSarah C Heilshorn
Jan 7, 2022·Journal of Anatomy·Samuele NegroMichela Rigoni

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