Visualizing the functional architecture of the endocytic machinery

Andrea PiccoMarko Kaksonen


Clathrin-mediated endocytosis is an essential process that forms vesicles from the plasma membrane. Although most of the protein components of the endocytic protein machinery have been thoroughly characterized, their organization at the endocytic site is poorly understood. We developed a fluorescence microscopy method to track the average positions of yeast endocytic proteins in relation to each other with a time precision below 1 s and with a spatial precision of ~10 nm. With these data, integrated with shapes of endocytic membrane intermediates and with superresolution imaging, we could visualize the dynamic architecture of the endocytic machinery. We showed how different coat proteins are distributed within the coat structure and how the assembly dynamics of N-BAR proteins relate to membrane shape changes. Moreover, we found that the region of actin polymerization is located at the base of the endocytic invagination, with the growing ends of filaments pointing toward the plasma membrane.


Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Doyle, D Botstein
Nov 15, 1996·The Journal of Biological Chemistry·F TebarTomas Kirchhausen
May 14, 1999·Current Biology : CB·D WinterRong Li
Nov 13, 1999·Nature Cell Biology·I GaidarovJ H Keen
Jan 29, 2000·The Journal of Cell Biology·T LechlerRong Li
Nov 1, 2001·Annual Review of Cell and Developmental Biology·F M BrodskyD E Wakeham
Aug 29, 2002·Nature Cell Biology·Christien J MerrifieldWolfhard Almers
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Jun 20, 2003·Current Biology : CB·Avital A RodalDavid G Drubin
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Jul 13, 2004·Trends in Cell Biology·Christien J Merrifield
Sep 3, 2004·Current Biology : CB·Gudrun A Jonsdottir, Rong Li
Jul 27, 2005·Journal of Structural Biology·I F Sbalzarini, P Koumoutsakos
Oct 15, 2005·Science·Jian-Qiu Wu, Thomas D Pollard
Nov 1, 2005·Biophysical Journal·L Stirling ChurchmanJames A Spudich
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Gang RenAlan L Munn
May 23, 2006·Nature Cell Biology·Ajit P JoglekarE D Salmon
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jian LiuGeorge Oster
Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Tadaomi Takenawa, Shiro Suetsugu
Jan 8, 2008·PLoS Biology·Brian J GallettaJohn A Cooper
Mar 19, 2008·The Journal of Cell Biology·Fatima-Zahra IdrissiMaría-Isabel Geli
Mar 26, 2009·Annual Review of Biochemistry·Gary J Doherty, Harvey T McMahon
Jul 15, 2009·Nature Cell Biology·Soheil Aghamohammadzadeh, Kathryn R Ayscough
Oct 20, 2009·Trends in Cell Biology·Tom Kirchhausen
Apr 7, 2010·Nature Methods·Carlas S SmithKeith A Lidke
Jun 12, 2010·Current Opinion in Cell Biology·Elizabeth Conibear
Jul 1, 2010·Molecular Biology of the Cell·Vladimir SirotkinThomas D Pollard
Jul 9, 2010·Molecular Biology of the Cell·Ji-Young YounBrenda Andrews
Oct 5, 2010·Current Protocols in Molecular Biology·Arthur EdelsteinNico Stuurman
Mar 30, 2011·PLoS Biology·Marcus J TaylorChristien J Merrifield
Jul 5, 2011·Current Biology : CB·Agnieszka CollinsTatyana Svitkina
Jul 28, 2011·Chembiochem : a European Journal of Chemical Biology·Xiaoli SunIvan R Corrêa
Aug 16, 2011·Nature Cell Biology·Steeve BoulantTomas Kirchhausen
Aug 19, 2011·Molecular Biology of the Cell·Douglas R BoettnerSandra K Lemmon
Sep 15, 2011·PloS One·Anton KhmelinskiiMichael Knop
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takuma KishimotoDavid G Drubin
Oct 25, 2011·Trends in Cell Biology·Jasper Weinberg, David G Drubin
Nov 16, 2011·Traffic·Iwona I Smaczynska-de RooijKathryn R Ayscough
Nov 16, 2011·The Journal of Cell Biology·Josh LawrimoreE D Salmon
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Benoît SorreAurélien Roux
Dec 23, 2011·Molecular Biology of the Cell·Susheela Y CarrollDavid G Drubin
Dec 24, 2011·Nature Cell Biology·Douglas R BoettnerSandra K Lemmon
Jun 6, 2012·Annual Review of Biochemistry·Olivia L MoorenJohn A Cooper
Jun 26, 2012·Nature Biotechnology·Anton KhmelinskiiMichael Knop
Aug 7, 2012·Cell·Emanuele CocucciTom Kirchhausen
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michal SkruznyMarko Kaksonen
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fatima-Zahra IdrissiMaría Isabel Geli
Sep 24, 2013·Cell Reports·Hongxia ZhaoPekka Lappalainen
Dec 11, 2013·The Journal of Cell Biology·Camilla Godlee, Marko Kaksonen
Jun 30, 2014·Methods in Cell Biology·Markus MundJonas Ries


Mar 24, 2016·The Journal of Cell Biology·Serge Dmitrieff, François Nédélec
Mar 31, 2016·Biophysical Journal·Xinxin WangA E Carlsson
Feb 6, 2016·Traffic·Florence Marie-AnaïsFlorence Niedergang
Nov 12, 2015·Developmental Cell·Eric B LewellynDavid G Drubin
Oct 31, 2015·PLoS Computational Biology·Serge Dmitrieff, François Nédélec
Mar 28, 2017·Nature Cell Biology·Kem A SochackiJustin W Taraska
Apr 17, 2016·Journal of Cell Science·Rebecca LuYidi Sun
Feb 8, 2018·Nature Reviews. Molecular Cell Biology·Marko Kaksonen, Aurélien Roux
Dec 8, 2017·Molecular Biology of the Cell·Rajesh ArasadaThomas D Pollard
Sep 20, 2017·The Journal of Cell Biology·Frederic DasteJennifer L Gallop
Oct 21, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marco M ManniAlenka Čopič
Dec 15, 2017·PLoS Computational Biology·Xinxin Wang, A E Carlsson
Jun 1, 2018·Molecular Biology of the Cell·Andrea PiccoMarko Kaksonen
Jul 15, 2018·FEBS Letters·Michael M LacyJulien Berro
Jul 26, 2018·Molecular Biology of the Cell·H AlimohamadiPadmini Rangamani
Jun 16, 2016·F1000Research·Eric A SheldenJonathan C R Jones
May 14, 2020·Molecular Systems Biology·Michal SkruznyVictor Sourjik
Aug 20, 2019·Nature Communications·Anbang DaiHong-Wei Wang
Jan 28, 2017·Proceedings of the National Academy of Sciences of the United States of America·Julian E HassingerPadmini Rangamani
May 31, 2018·PLoS Computational Biology·Rui Ma, Julien Berro
Sep 5, 2018·Journal of Cell Science·Xingyun QiDaniël Van Damme
Jun 27, 2019·Molecular Biology of the Cell·Masoud NickaeenBoris M Slepchenko
Aug 9, 2019·Journal of Cell Science·Cameron Dale MacQuarrieVladimir Sirotkin
Apr 22, 2018·Biochemical Society Transactions·Claudia Hinze, Emmanuel Boucrot
Jan 20, 2019·The Journal of Cell Biology·Ross T A Pedersen, David G Drubin
Jun 18, 2017·Physical Review. E·D J TwetenA E Carlsson
Oct 15, 2020·The Journal of Cell Biology·Ross T A PedersenDavid G Drubin
Sep 15, 2020·Current Opinion in Cell Biology·M M Kessels, Britta Qualmann
Nov 4, 2015·Journal of Cell Science·Violaine MoreauFrédéric Saltel
Apr 8, 2021·Biology of the Cell·Antoine AllardClement Campillo

Methods Mentioned

electron microscopy
superresolution microscopy
fluorescence recovery after photobleaching

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.