Vital role of protein kinase C-related kinase in the formation and stability of neurites during hypoxia.

Journal of Neurochemistry
Bettina ThauererGabriele Baier-Bitterlich

Abstract

Exposure of pheochromocytoma cells to hypoxia (1% O(2)) favors differentiation at the expense of cell viability. Additional incubation with nerve growth factor (NGF) and guanosine, a purine nucleoside with neurotrophin characteristics, rescued cell viability and further enhanced the extension of neurites. In parallel, an increase in the activity of protein kinase C-related kinase (PRK1), which is known to be involved in regulation of the actin cytoskeleton, was observed in hypoxic cells. NGF and guanosine further enhanced PRK1 in normoxic and hypoxic cells. To study the role of PRK1 during cellular stress response and neurotrophin-mediated signaling, pheochromocytoma cells were transfected with small interfering RNA directed against PRK1. Loss of functional PRK1 initiated a significant loss of viability and inhibited neurite formation. SiRNA-mediated knockdown of PRK1 also completely stalled guanosine-mediated neuroprotective effects. Additionally, the F-actin-associated cytoskeleton and the expression of the plasticity protein growth associated protein-43 were disturbed upon PRK1 knockdown. A comparable dependency of neurite formation and growth associated protein-43 immunoreactivity on functional PRK1 expression was observed ...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Dec 1, 1991·Stroke; a Journal of Cerebral Circulation·Y UemuraM F Beal
Jan 1, 1989·Annual Review of Neuroscience·J H Skene
Feb 1, 1985·The Journal of Cell Biology·M E Hatten
Apr 16, 1982·Neuroscience Letters·T ZetterströmB B Fredholm
Feb 1, 1996·Naunyn-Schmiedeberg's Archives of Pharmacology·I van der PloegB B Fredholm
Aug 1, 1996·The American Journal of Physiology·W H ZhuD E Millhorn
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·H MukaiY Ono
Feb 1, 1997·Current Opinion in Neurobiology·M E HattenN Heintz
Jun 6, 1998·The Journal of Physiology·S KobayashiD E Millhorn
Jun 26, 1998·The Biochemical Journal·H Mellor, P J Parker
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M TakahashiY Ono
Oct 13, 1998·Alzheimer Disease and Associated Disorders·M P RathboneR Huang
Feb 5, 1999·Naunyn-Schmiedeberg's Archives of Pharmacology·G ArslanB B Fredholm
Oct 19, 1999·Brain Research. Brain Research Reviews·R Berger, Y Garnier
Jan 11, 2000·Behavioural Brain Research·B F El-Khodor, P Boksa
Jun 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peng ChenLarry I Benowitz
Aug 22, 2002·Science's STKE : Signal Transduction Knowledge Environment·K A SetaD E Millhorn
Sep 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nina IrwinLarry I Benowitz
Mar 27, 2003·Progress in Neuro-psychopharmacology & Biological Psychiatry·Seth Love
Dec 4, 2003·Bioorganic & Medicinal Chemistry·Ugo TraversaRoberto Pellicciari
May 19, 2004·European Journal of Cell Biology·Karl BöcklingerGabriele Baier-Bitterlich
Jan 5, 2005·Genes & Development·Eve-Ellen GovekLinda Van Aelst
Apr 22, 2005·Neurochemistry International·Bettina TomaselliGabriele Baier-Bitterlich
Jul 16, 2005·Neurochemical Research·Ana Paula HornChristianne Salbego
Feb 14, 2006·Methods in Enzymology·Annette GärtnerGiovanna Lalli
Jan 5, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Iolanda D'AlimonteRenata Ciccarelli
Mar 31, 2007·Cell Death and Differentiation·B B Fredholm
Sep 8, 2007·Neurochemistry International·Scheyla P B OleskoviczCarla I Tasca
Sep 22, 2007·Pharmacology & Therapeutics·André P SchmidtDiogo O Souza
Nov 17, 2007·The Journal of Biological Chemistry·Rakhee ModhaHelen R Mott

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Citations

Feb 18, 2012·Journal of Neurochemistry·Bettina ThauererGabriele Baier-Bitterlich
Mar 24, 2016·Purinergic Signalling·Luis E B BettioAna Lúcia S Rodrigues
Jan 30, 2013·Neurochemistry International·Philipp AlbrechtAxel Methner
Oct 5, 2016·Aging and Disease·Débora LanznasterCarla I Tasca
Mar 2, 2018·The Journal of Clinical Investigation·Stephanie zur NeddenGabriele Baier-Bitterlich
Nov 22, 2018·Frontiers in Cellular Neuroscience·Carla I TascaFrancisco Ciruela
Jul 3, 2021·International Journal of Molecular Sciences·Karol ChojnowskiRyszard T Smolenski

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