PMID: 9649586Jul 3, 1998Paper

Vitamin A is required for regulation of polymeric immunoglobulin receptor (pIgR) expression by interleukin-4 and interferon-gamma in a human intestinal epithelial cell line

The Journal of Nutrition
J SarkarC B Stephensen

Abstract

The secretory immunoglobulin A (IgA) antibody response to infections of mucosal surfaces requires transport of IgA from the basal to apical surface of mucosal epithelial cells by a specific transport protein, the polymeric immunoglobulin receptor (pIgR). We have tested the hypothesis that the vitamin A metabolite all-trans retinoic acid (RA) is required for the regulation of pIgR expression by the cytokines interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) in HT-29 cells, a well-differentiated human epithelial cell line derived from a colonic carcinoma. pIgR expression is upregulated by IFN-gamma and IL-4 when HT-29 cells are grown in normal media, but this upregulation was significantly lower when cells were grown in vitamin A-depleted media. Treatment with RA at concentrations from 10(-9) to 10(-5) mol/L restored normal levels of pIgR expression. The percentages of cells expressing cell-surface pIgR after 24, 48 and 72 h of treatment with RA, IL-4 and IFN-gamma were 66 +/- 10, 90 +/- 5 and 92 +/- 1, respectively, significantly higher than the percentages seen without RA treatment, which were 32 +/- 2.3, 72 +/- 1.2 and 30 +/- 7, respectively. In addition, the intensity of fluorescence of pIgR-positive cells was significant...Continue Reading

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Citations

Jun 9, 2007·Scandinavian Journal of Medicine & Science in Sports·F KimuraI Kono
Dec 22, 2012·Beneficial Microbes·P Brandtzaeg
May 12, 2016·Annual Review of Immunology·Alexandre Larange, Hilde Cheroutre
Apr 30, 2016·Critical Reviews in Food Science and Nutrition·Elina Manusevich WisemanRam Reifen
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zeina JaffarKevan Roberts
Mar 11, 2004·Clinical and Experimental Immunology·N Takenouchi-OhkuboI Moro
Mar 7, 2021·International Journal of Molecular Sciences·Hao Wei, Ji-Yang Wang
Dec 12, 2001·Medical Hypotheses·T Binstock
May 21, 2003·International Journal of Medical Microbiology : IJMM·Per Brandtzaeg
Sep 23, 2008·Seminars in Immunology·J Rodrigo Mora, Ulrich H von Andrian
Aug 6, 2011·European Journal of Pharmacology·Per Brandtzaeg

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