Vitamin A supplementation improves the intestinal mucosal barrier and facilitates the expression of tight junction proteins in rats with diarrhea

Nutrition
Lu XiaoJie Chen

Abstract

The aim of this study is to investigate the specific effects of vitamin A (VA) on diarrhea in rats and its potential targets to protect the intestinal mucosa. Specific pathogen-free Sprague Dawley rats were fed a VA deficient (VAD) or VA normal (VAN) diet for 4 wk. Then, half of the VAN rats were treated with a VAN diet and the other half with a lactose VAN diet. VAD rats were randomly assigned to one of four groups and fed a VAD diet, lactose VAD diet, VAN diet with VA supplementation (VAS) via daily intragastric administration, or a lactose VAN diet with daily VAS. Rat weight and degree of diarrhea were evaluated daily. After 15 d, the serum retinol level was measured by high-performance liquid chromatography, and the serum diamine oxidase (DAO) and zonulin concentrations were analyzed by enzyme-linked immunosorbent assays. The small intestine mucosal pathology was observed by hematoxylin and eosin staining. Western blotting was performed to detect the protein expression levels of occludin and claudin-1 in the intestinal mucosa, and the zonula-occludens 1 expression was assessed using immunohistochemistry. VAD limited weight gain in rats and increased the degree of diarrhea. The serum retinol levels and the level of tight jun...Continue Reading

Citations

Mar 29, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Takuya Suzuki
Sep 23, 2020·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Huaijin ChengXueyong Shen
Sep 6, 2020·Microorganisms·Katharina BeckNaoko Satoh-Takayama
Sep 30, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Affonso Flávio Jorge MussolinoUenis Tannuri

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