Vitamin B12 Deficiency Induces Imbalance in Melanocytes Homeostasis-A Cellular Basis of Hypocobalaminemia Pigmentary Manifestations

International Journal of Molecular Sciences
Zuzanna RzepkaDorota Wrześniok

Abstract

Vitamin B12 deficiency causes significant changes in cellular metabolism leading to various clinical symptoms, such as hematological, psychiatric, and neurological disorders. We hypothesize that skin pigmentation disorders may be a diagnostically important manifestation of vitamin B12 deficiency, however the cellular and molecular mechanisms underlying these effects remain unknown. The aim of this study was to examine the effect of vitamin B12 deficiency on melanocytes homeostasis. Hypocobalaminemia in vitro model was developed by treating epidermal melanocytes with synthesized vitamin B12 antagonist-hydroxycobalamin(c-lactam). The cells were examined using immunoenzymatic, spectrophotometric, and fluorimetric assays as well as image cytometry. Significant melanogenesis stimulation-the increase of relative melanin content and tyrosinase activity up to 131% and 135%, respectively-has been indicated. Cobalamin-deficient cells displayed the elevation (by 120%) in reactive oxygen species level. Moreover, the redox status imbalance was stated. The study provided a scientific evidence for melanocytes homeostasis disturbance under hypocobalaminemia, thus indicating a significant element of the hyperpigmentation mechanism due to vitami...Continue Reading

References

Apr 1, 1991·The Journal of Clinical Investigation·S P StablerR H Allen
Jul 5, 2001·The Journal of Dermatology·K MoriA Kukita
Apr 16, 2003·Kidney International. Supplement·Alessandra F PernaNatale G De Santo
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman
Jan 19, 2005·The Journal of Investigative Dermatology·Andrzej SlominskiDesmond J Tobin
Aug 9, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Neetu TyagiSuresh C Tyagi
Oct 24, 2007·European Journal of Dermatology : EJD·Shiro Niiyama, Hideki Mukai
Jun 5, 2008·Antioxidants & Redox Signaling·Dunyaporn TrachoothamPeng Huang
Nov 13, 2008·Antioxidants & Redox Signaling·Nicolas BrandesUrsula Jakob
Nov 26, 2008·European Heart Journal·Charalambos AntoniadesChristodoulos Stefanadis
Jan 15, 2010·Methods in Molecular Biology·Evgeniy Eruslanov, Sergei Kusmartsev
Aug 13, 2011·Pigment Cell & Melanoma Research·Andrzej SlominskiJohn Pawelek
Feb 19, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dorota WrześniokEwa Buszman
May 30, 2013·Chemical Society Reviews·Keith o óProinsiasDorota Gryko
Jun 28, 2013·Journal of Cellular Biochemistry·Dorota WrześniokEwa Buszman
Jul 11, 2013·Case Reports in Hematology·Rabia CherqaouiJames Williams
Jul 23, 2013·Molecular and Cellular Biochemistry·Dorota WrześniokEwa Buszman
Nov 20, 2013·Nutrients·Chiara BrianiFausto Adami
Nov 20, 2013·Indian Journal of Endocrinology and Metabolism·Ritesh Kumar AgrawalaAnoj Kumar Baliarsinha
Dec 7, 2013·Cutaneous and Ocular Toxicology·Nihat DemirSekibe Zehra Doğan
Apr 22, 2015·Clinical Diabetes : a Publication of the American Diabetes Association·Matthew J Zdilla
May 4, 2015·Journal of Photochemistry and Photobiology. B, Biology·Jakub RokDorota Wrześniok
May 5, 2017·Methods in Molecular Biology·Konstantin PräbstHolger Hübner
Dec 19, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jakub RokEwa Buszman
Feb 13, 2018·The Journal of Investigative Dermatology·Andrzej T SlominskiRalf Paus

❮ Previous
Next ❯

Citations

Mar 3, 2019·Nutrients·Erik E van de LagemaatEllen G H M van den Heuvel
Nov 28, 2019·Cells·Zuzanna RzepkaDorota Wrześniok
Sep 22, 2020·Chemistry : a European Journal·Bernhard Kräutler
Aug 7, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Selçuk Kaplan, Ahmet Türk

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
enzyme-linked immuno-sorbent assay
Protein Assay
Assay

Software Mentioned

ChemoMetec
STATISTICA
NucleoView NC - [UNK]
[UNK]

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The American Journal of Medicine
Kshitij MankadIndran Davagnanam
Toxicology in Vitro : an International Journal Published in Association with BIBRA
Michał OtrębaEwa Buszman
Asian Journal of Psychiatry
Shiva Shanker Reddy MukkuSantosh Kumar Chaturvedi
© 2022 Meta ULC. All rights reserved