Vitamin C increases 5-hydroxymethylcytosine level and inhibits the growth of bladder cancer

Clinical Epigenetics
Ding PengLiqun Zhou

Abstract

5-Hydroxymethylcytosine (5hmC) is converted from 5-methylcytosine (5mC) by a group of enzymes termed ten-eleven translocation (TET) family dioxygenases. The loss of 5hmC has been identified as a hallmark of most types of cancer and is related to tumorigenesis and progression. However, the role of 5hmC in bladder cancer is seldom investigated. Vitamin C was recently reported to induce the generation of 5hmC by acting as a cofactor for TET dioxygenases. In this study, we explored the role of 5hmC in bladder cancer and the therapeutic efficacy of vitamin C in increasing the 5hmC pattern. 5hmC was decreased in bladder cancer samples and was related to patient overall survival. Genome-wide mapping of 5hmC in tumor tissues and vitamin C-treated bladder cancer cells revealed that 5hmC loss was enriched in cancer-related genes and that vitamin C treatment increased 5hmC levels correspondingly. Vitamin C treatment shifted the transcriptome and inhibited the malignant phenotypes associated with bladder cancer cells in both in vitro cell lines and in vivo xenografts. This study provided mechanistic insights regarding the 5hmC loss in bladder cancer and a rationale for exploring the therapeutic use of vitamin C as a potential epigenetic tr...Continue Reading

References

Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Nov 25, 2003·The New England Journal of Medicine·James G Herman, Stephen B Baylin
Feb 19, 2008·Nature Chemical Biology·Christoph Loenarz, Christopher J Schofield
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Sep 24, 2011·Nature Reviews. Cancer·Stephen B Baylin, Peter A Jones
Sep 18, 2012·Cell·Christine Guo LianYujiang G Shi
Sep 21, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Jean Cadet, J Richard Wagner
Jun 4, 2014·CA: a Cancer Journal for Clinicians·Carol E DeSantisAhmedin Jemal
May 16, 2015·Clinical Epigenetics·Christopher B GustafsonGaofeng Wang
Sep 13, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sarah E B TaylorNidhi Bhutani
Jun 28, 2016·Lancet·Ashish M KamatWassim Kassouf
Jul 5, 2016·European Urology·J Alfred WitjesMaria J Ribal
Aug 18, 2016·Nature·Bernard ThienpontDiether Lambrechts
Aug 31, 2016·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiuPeter A Jones
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Timothy Alexander HoreWolf Reik
Aug 22, 2017·Nature·Michalis AgathocleousSean J Morrison

❮ Previous
Next ❯

Citations

Apr 17, 2019·Bioscience Reports·Songsong TengChengqing Yi
Dec 18, 2019·Essays in Biochemistry·Ksenia SkvortsovaPhillippa Taberlay
Aug 23, 2020·Antioxidants·Laura Bordoni, Rosita Gabbianelli
Oct 19, 2019·British Journal of Pharmacology·Megan BeetchBarbara Stefanska
Oct 22, 2020·Critical Reviews in Food Science and Nutrition·Ankita KumariRajeev Kapila
Mar 4, 2021·Nutrients·Anna Zasowska-NowakAleksandra Ciałkowska-Rysz
Apr 4, 2021·International Journal of Molecular Sciences·Carmelo GurnariValeria Visconte
Apr 1, 2021·Free Radical Biology & Medicine·Stine Ulrik MikkelsenKirsten Grønbæk
May 22, 2021·Frontiers in Genetics·John P BrabsonLuisa Cimmino
Aug 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Maike BensbergColm E Nestor
Dec 24, 2020·Nutrients·Kinga LinowieckaAnna A Brożyna
Jan 6, 2022·Journal of the American Society of Nephrology : JASN·Zihui YuWeimin Ci

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSM1059457

Methods Mentioned

BETA
dot blot
immunoprecipitation
hMeDIP-seq
xenografts
xenograft
RNA-seq
dot
ChIP-Seq
PCR
FACS

Software Mentioned

R
package
bowtie2
Database for Annotation , Visualization , and Integrated Disco...
ChIP
SPSS
Tophat
CEAS
MACS
GSEA

Related Concepts

Related Feeds

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.