Vitamin C regulates keratinocyte viability, epidermal barrier, and basement membrane in vitro, and reduces wound contraction after grafting of cultured skin substitutes

The Journal of Investigative Dermatology
Steven T BoyceG D Warden

Abstract

Cultured skin substitutes have become useful as adjunctive treatments for excised, full-thickness burns, but no skin substitutes have the anatomy and physiology of native skin. Hypothetically, deficiencies of structure and function may result, in part, from nutritional deficiencies in culture media. To address this hypothesis, vitamin C was titrated at 0.0, 0.01, 0.1, and 1.0 mM in a cultured skin substitute model on filter inserts. Cultured skin substitute inserts were evaluated at 2 and 5 wk for viability by incorporation of 5-bromo-2'-deoxyuridine (BrdU) and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion. Subsequently, cultured skin substitute grafts consisting of cultured human keratinocytes and fibroblasts attached to collagen-glycosaminoglycan substrates were incubated for 5 wk in media containing 0.0 mM or 0.1 mM vitamin C, and then grafted to athymic mice. Cultured skin substitutes (n = 3 per group) were evaluated in vitro at 2 wk of incubation for collagen IV, collagen VII, and laminin 5, and through 5 wk for epidermal barrier by surface electrical capacitance. Cultured skin substitutes were grafted to full-thickness wounds in athymic mice (n = 8 per group), evaluated for surface elec...Continue Reading

References

Jul 12, 1979·The New England Journal of Medicine·D J ProckopN A Guzman
Sep 30, 1975·Annals of the New York Academy of Sciences·M J Barnes
Jul 5, 1979·The New England Journal of Medicine·D J ProckopN A Guzman
Oct 20, 1989·JAMA : the Journal of the American Medical Association·J F HansbroughT J Foreman
Aug 16, 1984·The New England Journal of Medicine·G G GallicoH Green
Mar 1, 1981·Plastic and Reconstructive Surgery·E BellD J Buttle
May 1, 1995·The Journal of Cell Biology·C H StreuliM J Bissell
Jan 1, 1995·Skin Pharmacology : the Official Journal of the Skin Pharmacology Society·M Ponec, J Kempenaar
Jan 1, 1995·Archives of Dermatological Research·M PonecH Jansen
Jun 1, 1997·The British Journal of Dermatology·F Berthod, O Damour
Sep 12, 1998·The Journal of Investigative Dermatology. Symposium Proceedings·J VicanováM Ponec
Apr 26, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A P SuppS T Boyce
Apr 1, 1997·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·M D HarrigerI A Holder
Oct 1, 1995·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·M J GoretskyS T Boyce
Jan 1, 1996·Tissue Engineering·S T Boyce

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Citations

Apr 11, 2008·Tissue Engineering. Part a·Balaji KalyanaramanSteven T Boyce
Oct 1, 2004·BMC Dermatology·Kirsten SauermannHorst Wenck
Feb 16, 2016·Contact Dermatitis·Pieter-Jan CoenraadsMarie-Louise A Schuttelaar
Apr 20, 2010·Journal of Dermatological Science·Hitoshi Masaki
Jul 22, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Patrick J HaydenMitchell Klausner
Jan 8, 2004·The Journal of Investigative Dermatology·Woan-Ruoh LeeJia-You Fang
Feb 13, 2007·European Journal of Cell Biology·Karsten BoehnkeHans-Jürgen Stark
Aug 12, 2004·International Journal of Dermatology·Liliana Elizabeth Espinal-PerezJuan Pablo Castanedo-Cazares
Jul 22, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Kevin L McFarlandDorothy M Supp
Oct 22, 2008·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Namrata D BaraiGerald B Kasting
Jan 16, 2007·Experimental Dermatology·Montserrat SerraLluís Ferrer
Jul 18, 2006·Experimental Dermatology·Claire MarionnetFrançoise Bernerd
Jun 8, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Steven T BoyceDorothy M Supp
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ulla StumpfMartin Scholz
Mar 19, 2014·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Abigail M WojtowiczDolores Baksh
Jun 15, 2004·Journal of Dermatological Science·Hyun-Joo ChoKyoung-Chan Park
Aug 18, 2009·Chemico-biological Interactions·Robert H RiceCort Anastasio
Dec 16, 2005·The Journal of Investigative Dermatology·Andrea K SmileyDorothy M Supp
Aug 19, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Christopher LloydSteven Boyce
May 16, 2003·Current Biology : CB·Christelle CorauxDaniel Aberdam
Sep 14, 2012·Oxidative Medicine and Cellular Longevity·Hye-Ryung ChoiKyoung-Chan Park
Jun 4, 2015·Revista latino-americana de enfermagem·Mariano del Sol CalderonFelipe Ocharan Torre
Jul 19, 2005·Clinics in Dermatology·Dorothy M Supp, Steven T Boyce
Mar 16, 2017·Plastic and Reconstructive Surgery. Global Open·Dorothy M SuppBruce D Hammock
May 25, 2006·Cell Transplantation·Steven T BoyceHoracio L Rodriguez-Rilo
Aug 15, 2017·Nutrients·Juliet M PullarMargreet C M Vissers
Jul 24, 2013·Journal of Burn Care & Research : Official Publication of the American Burn Association·Marc G JeschkeUNKNOWN ABA Organization and Delivery of Burn Care Committee
Jul 13, 2016·Journal of Burn Care & Research : Official Publication of the American Burn Association·Steven T BoyceRichard J Kagan
Apr 20, 2018·Advances in Skin & Wound Care·Julian-Dario RembeEwa Klara Stuermer

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