Abstract
Differentiating chick limb-bud mesenchymal cell micro-mass cultures routinely mineralize in the presence of 10% fetal calf serum, antibiotics, 4 mM inorganic phosphate (or 2.5 mM beta-glycerophosphate), 0.3 mg/ml glutamine and either 25 microg/ml vitamin C or 5-12 microg/ml vitamin C-sulfate. The failure of these cultures to produce a mineralized matrix (assessed by electron microscopy, 45Ca uptake and Fourier transform infrared microscopy) led to the evaluation of each of these additives. We report here that the "stable" vitamin C-sulfate (ascorbic acid-2-sulfate) causes increased sulfate incorporation into the cartilage matrix. Furthermore, the release of sulfate from the vitamin C derivative appears to be responsible for the inhibition of mineral deposition, as demonstrated in cultures with equimolar amounts of vitamin C and sodium sulfate.
References
Sep 30, 1975·Annals of the New York Academy of Sciences·B M TolbertE M Baker
Apr 8, 1976·Biochimica Et Biophysica Acta·A L FluhartyH Kihara
Jan 1, 1976·Connective Tissue Research·E R Schwartz, L Adamy
Apr 7, 1975·Biochimica Et Biophysica Acta·S S Shapiro, J P Poon
Oct 1, 1977·Developmental Biology·P B AhrensR S Reiter
Dec 1, 1992·Journal of Nutritional Science and Vitaminology·K HitomiN Tsukagoshi
Dec 1, 1992·Calcified Tissue International·A L BoskeyI Binderman
Jan 1, 1991·Bone·A L BoskeyI Binderman
Dec 1, 1991·Calcified Tissue International·A L BoskeyA Hjerpe
Oct 1, 1988·Journal of Neurochemistry·H Wilgus, R Roskoski
Mar 1, 1988·Journal of Biochemical and Biophysical Methods·P M van der KraanL B van de Putte
Oct 17, 1985·Biochimica Et Biophysica Acta·H I RoachJ R Shearer
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Jan 1, 1981·Acta Anatomica·B K Hall
May 1, 1994·Calcified Tissue International·D D DeanB D Boyan
Mar 1, 1996·Calcified Tissue International·A L BoskeyI Binderman
Dec 6, 1996·Molecular and Cellular Biochemistry·Y ToriiN Tsukagoshi
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Mar 15, 1997·Journal of Cellular Biochemistry·A L BoskeyS B Doty
Sep 1, 1997·Journal of Cellular Biochemistry·P S LeboyR A Venezian
Oct 6, 1997·Calcified Tissue International·A L BoskeyL Rosenberg
Aug 24, 1999·Cell Biology International·B FermorJ Gage
Nov 7, 1999·Histochemistry and Cell Biology·H Tullberg-Reinert, G Jundt
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON