Oct 1, 1977

Vitamin D binding factors in bovine blood

Journal of Dairy Science
B W HollisH R Conrad

Abstract

Both 25 [25,26-hydrogen-3] hydroxycholecalciferol and [1alpha,2alpha-hydrogen-3] cholecalciferol were added to bovine plasma in vitro. Analysis by gel-filtration and ion exchange chromatography, electrophoresis, ultracentrifugation, competitive binding specificity studies, and plasma stripping showed that vitamin D circulated with a protein of alpha-globulin mobility. This globulin had a much higher affinity for 25-hydroxycholecalciferol while vitamin D3 appeared to be associated first with an alpha-lipoprotein and with time because associated with the alpha-globulin. This alpha-globulin had a molecular weight of approximately 70,000 as determined by gel-filtration. Cholecalciferol appeared to bind tightly to the alpha-lipoprotein and resisted being stripped from the plasma. Thus, alpha-globulin appears to be the major carrier of vitamin D in the blood while the alpha-lipoprotein may aid in the transfer of cholecalciferol from the gut to the liver via the lymph system.

  • References8
  • Citations1

References

  • References8
  • Citations1

Citations

Mentioned in this Paper

Alfacalcidol
Filtration
Ergocalciferol
Bos taurus
Cholecalciferol
Globulins
Lymph
Vitamin D
Staphylococcal Protein A
High Density Lipoproteins

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