Vitamin-D-dependent transcriptional regulation of the intestinal plasma membrane calcium pump

Biochemical and Biophysical Research Communications
T L PannabeckerR H Wasserman

Abstract

The vitamin D hormone, 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH)2D3 intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH)2D3 regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.

Citations

Nov 4, 2000·Biochemical and Biophysical Research Communications·P GlendenningE E Strehler
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·S J Van CromphautG Carmeliet
Dec 5, 2008·Nutrition Reviews·Daniel R Schmidt, David J Mangelsdorf
Jul 19, 2005·Journal of Mammary Gland Biology and Neoplasia·Ronald L HorstTimothy A Reinhardt
Nov 16, 2010·Cell Calcium·Michael F RitchieJonathan Soboloff
Aug 7, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Arie Bar
Dec 3, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mirja R WilkensBernd Schröder
Oct 29, 1998·The Journal of Biological Chemistry·J B SafranM C Farach-Carson
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel
Oct 28, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Sylvia ChristakosJames C Fleet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.