Vitamin D enhances IL-1β secretion and restricts growth of Mycobacterium tuberculosis in macrophages from TB patients

International Journal of Mycobacteriology
Daniel EklundMaria Lerm

Abstract

The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis (TB), has rekindled the interest in the role of nutritional supplementation of micronutrients, such as vitamin D, as adjuvant treatment. Here, the growth of virulent MTB in macrophages obtained from the peripheral blood of patients with and without TB was studied. The H37Rv strain genetically modified to express Vibrio harveyi luciferase was used to determine the growth of MTB by luminometry in the human monocyte-derived macrophages (hMDMs) from study subjects. Determination of cytokine levels in culture supernatants was performed using a flow cytometry-based bead array technique. No differences in intracellular growth of MTB were observed between the different study groups. However, stimulation with 100nM 1,25-dihydroxyvitamin D significantly enhanced the capacity of hMDMs isolated from TB patients to control the infection. This effect was not observed in hMDMs from the other groups. The interleukin (IL)-1β and IL-10 release by hMDMs was clearly increased upon stimulation with 1,25-dihydroxyvitamin D. Furthermore, the 1,25-dihydroxyvitamin D stimulation also led to elevated levels of TNF-α (tumor necrosi...Continue Reading

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Citations

Dec 15, 2015·International Immunopharmacology·Wafa NouariMourad Aribi
Nov 25, 2017·International Journal of Mycobacteriology·Manik Retno WahyunitisariEko B Koendhori
Nov 2, 2019·Journal of Nutritional Science and Vitaminology·Qiuzhen WangEvert G Schouten
Oct 18, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Avinash PadhiAvinash Sonawane
Feb 11, 2020·Frontiers in Immunology·Jagadeeswara Rao MuvvaSusanna Brighenti
Jun 18, 2019·BioMed Research International·Muhammad M AslamM I Kamboh
Jan 14, 2017·Microbiology Spectrum·Elsa Du Bruyn, Robert John Wilkinson

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