Vitamin D inhibits lipopolysaccharide-induced inflammatory response potentially through the Toll-like receptor 4 signalling pathway in the intestine and enterocytes of juvenile Jian carp (Cyprinus carpio var. Jian)

The British Journal of Nutrition
Jun JiangYe Zhao

Abstract

The present study was conducted to investigate the anti-inflammatory effect of vitamin D both in juvenile Jian carp (Cyprinus carpio var. Jian) in vivo and in enterocytes in vitro. In primary enterocytes, exposure to 10 mg lipopolysaccharide (LPS)/l increased lactate dehydrogenase activity in the culture medium (P<0·05) and resulted in a significant loss of cell viability (P<0·05). LPS exposure increased (P<0·05) the mRNA expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-8), which was decreased by pre-treatment with 1,25-dihydroxyvitamin D (1,25D3) in a dose-dependent manner (P<0·05). Further results showed that pre-treatment with 1,25D3 down-regulated Toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (Myd88) and NF-κB p65 mRNA expression (P<0·05), suggesting potential mechanisms against LPS-induced inflammatory response. In vivo, intraperitoneal injection of LPS significantly increased TNF-α, IL-1β, IL-6 and IL-8 mRNA expression in the intestine of carp (P<0·05). Pre-treatment of fish with vitamin D3 protected the fish intestine from the LPS-induced increase of TNF-α, IL-1β, IL-6 and IL-8 mainly by downregulating TLR4, Myd88 and NF-κB p65 mRNA expression (P<0·05). These observation...Continue Reading

References

Oct 1, 1986·General and Comparative Endocrinology·M E HayesI W Henderson
Feb 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·L V AvioliJ S Chandler
Apr 10, 1999·The Journal of Biological Chemistry·J C ChowF Gusovsky
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A E MedvedevS N Vogel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Osamu TakeuchiShizuo Akira
Jul 18, 2002·Zeitschrift für Gastroenterologie·C Maaser, M F Kagnoff
Mar 13, 2003·Developmental and Comparative Immunology·Simon MacKenzieFrederick W Goetz
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadia GiarratanaLuciano Adorini
Nov 17, 2004·Trends in Biochemical Sciences·David E Prosser, Glenville Jones
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xiangyang DongMatthew D Griffin
Mar 2, 2006·American Journal of Physiology. Endocrinology and Metabolism·Jun SunYan Chun Li
Mar 28, 2006·Progress in Biophysics and Molecular Biology·P Lips
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip T LiuRobert L Modlin
Dec 9, 2008·Fish & Shellfish Immunology·Rebeca CerezuelaMa Angeles Esteban
Feb 28, 2009·International Immunology·Taro Kawai, Shizuo Akira
Sep 14, 2010·Fish & Shellfish Immunology·Jan H W M RomboutViswanath Kiron
Apr 14, 2012·Analytical Biochemistry·Marie-Aline PierrardFrédéric Silvestre
May 18, 2013·Nature Reviews. Immunology·Luke A J O'NeillAndrew G Bowie

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Citations

Feb 26, 2016·Developmental and Comparative Immunology·Carolina TafallaUwe Fischer
Mar 8, 2016·Nutrients·Charles S Hii, Antonio Ferrante
Mar 7, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Shubin LiuLiqiao Chen
Aug 28, 2021·Fish & Shellfish Immunology·Jiayu LiuMin Wan

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