PMID: 3754768Jun 13, 1986Paper

Vitamin D metabolites stimulate phosphatidylcholine transfer to renal brush-border membranes

Biochimica Et Biophysica Acta
B R KurnikK A Hruska

Abstract

The phosphatidylcholine content of both the intestinal and renal brush-border membranes and ion transport are affected by 1,25-dihydroxycholecalciferol (1,25(OH)2D3). To investigate the mechanism of this effect, liposomes were prepared containing self-quenching concentrations of fluorescent phospholipid derivatives. When these liposomes were incubated with rat renal brush-border membrane vesicles, an immediate increase in the relative fluorescence of N-4-nitrobenz-2-oxa-1,3-diazole phosphatidylcholine (NBD-PC) was detected, indicating transfer of NBD-PC into a non-quenched membrane. Addition of 1,25(OH)2D3 to the liposomes produced a dose-dependent stimulation of NBD-PC transfer to the acceptor brush-border membrane vesicles. Peripheral fluorescence was visible when the brush-border membrane vesicles were viewed with a fluorescent microscope. Using brush-border membrane vesicles from kidneys of vitamin D-deficient animals, quantitation of lipid transfer revealed a 1,25(OH)2D3 (10(-7) M) stimulation of NBD-PC transfer from 1.38 +/- 0.27 to 2.07 +/- 0.26 micrograms/h, and of PC transfer, assessed by vesicle phosphatidylcholine content, from 49.7 +/- 12 to 57.3 +/- 12 micrograms/mg protein per h (P less than 0.05). There was no si...Continue Reading

References

Jun 14, 1977·Biochemistry·Y BarenholzR D Carlson
Jul 11, 1985·The American Journal of Physiology·C S HyunM Field
Jul 11, 1985·Biochimica Et Biophysica Acta·B R Kurnik, K A Hruska
Feb 29, 1984·Biochimica Et Biophysica Acta·R P Holmes, N L Yoss
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

May 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D T BaranM F Holick
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Barsony, S J Marx
Mar 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·F BellemareC Malo

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