Vitamin D Receptor Expression in Dogs

Journal of Veterinary Internal Medicine
J A CartwrightR J Mellanby

Abstract

There is growing evidence linking low blood vitamin D concentration to numerous diseases in people and in dogs. Vitamin D influences cellular function by signaling through the vitamin D receptor (VDR). Little is known about which non-skeletal tissues express the VDR or how inflammation influences its expression in the dog. To define which non-skeletal canine tissues express the VDR and to investigate expression in inflamed small intestine. Thirteen non-skeletal tissues were collected prospectively from 6 control dogs. Thirty-five dogs diagnosed with a chronic enteropathy (CE) and 24 control dogs were prospectively enrolled and duodenal biopsies were evaluated for VDR expression. Prospective; blinded assessment of canine intestinal VDR. Dogs with CE were included once other identifiable causes of intestinal disease were excluded. Age matched controls were included with no intestinal clinical signs. VDR expression was assessed immunohistochemically in all samples, using a Rat IgG VDR monoclonal antibody. Quantitative real-time polymerase chain reaction (qPCR) was also used for duodenal biopsies. VDR expression as assessed by immunohistochemistry (IHC) was highest in the kidney, duodenum, skin, ileum and spleen, and weak in the co...Continue Reading

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Citations

Dec 6, 2019·Journal of Animal Physiology and Animal Nutrition·Rafael V A ZafalonMarcio A Brunetto
Nov 13, 2020·Journal of Veterinary Internal Medicine·Dylan N ClementsRichard J Mellanby
Apr 3, 2021·Research in Veterinary Science·Dylan N ClementsRichard J Mellanby
Apr 24, 2021·Journal of Nutritional Science·Rachel A KurzbardShiguang Yu
May 26, 2021·Peptides·C E Valdez-MiramontesB Rivas-Santiago
May 27, 2021·BMC Veterinary Research·R Sánchez-CéspedesY Millán
Jul 30, 2021·The Journal of Small Animal Practice·K E ClarkeR J Mellanby

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Methods Mentioned

BETA
biopsy
biopsies
nucleic acid folding
PCR

Software Mentioned

REST
RNALater
OligoAnalyser
Relative expression software tool ( REST )
Best Keeper
Basic Local Alignment Search Tool
Ensembl
Roche
Cell

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