PMID: 9442914Jan 27, 1998Paper

Vitamin D receptor in IMR-90 human fibroblasts and antiproliferative effect of 1,25-dihydroxyvitamin D3

Biochemistry and Molecular Biology International
M StioC Treves

Abstract

This study examines the effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], serum or forskolin on the proliferation of IMR-90 fetal lung fibroblasts and demonstrates, for the first time, the presence of 1,25(OH)2D3 receptor (VDR) in this cell line. In quiescent, subconfluent cultures neither the treatment with 100 nM 1,25(OH)2D3 nor that with 50 microM forskolin influenced proliferation, while a significant increase was observed after incubation of the cells with 10% fetal bovine serum. Either cell number, determined on growing IMR-90 human fibroblasts after 48 or 72 h incubation with 100 nM 1,25(OH)2D3 or [3H]thymidine incorporation (24, 48 or 72 h incubation) significantly decreased, while protein content per cell increased. Northern blot analysis revealed the expression of the VDR gene, the VDR mRNA bands being prominent in 1,25(OH)2D3, serum or forskolin treated fibroblasts. VDR mRNA levels slightly decreased, when growing fibroblasts were exposed to 100 nM 1,25(OH)2D3 for 48 or 72 h.

Citations

Nov 26, 2011·The Proceedings of the Nutrition Society·Augusto A Litonjua
Apr 15, 2009·Current Opinion in Allergy and Clinical Immunology·Augusto A Litonjua
May 21, 2011·American Journal of Respiratory and Critical Care Medicine·Scott T Weiss, Augusto A Litonjua
Jan 12, 2016·International Forum of Allergy & Rhinology·William W CarrollJennifer K Mulligan
Jan 28, 2015·The Journal of Steroid Biochemistry and Molecular Biology·James YawnJennifer K Mulligan
Jun 17, 2008·Otolaryngologia polska. The Polish otolaryngology·Beata Rostkowska-NadolskaMarcin Fraczek

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